Invitrogen™ BioPrime™ Total Genomic Labeling Module

Description

The BioPrime Total Genomic Labeling Module is part of the system that offers users the following advantages:

• Higher signal to noise with Alexa Fluor™ 3 and 5 dyes
• Less channel bias due to novel reaction formulation
• Widest range of input material (50ng - 3μg)
• Simplified workflow with master mix formulation

This complete, all-inclusive kit is optimized to work across the widest range of sample input material with no need for pre-amplification. Highly concentrated exo-Klenow fragment of DNA polymerase I in a component-limited reaction allows for consistent, robust DNA yields of approx. 8μg with as little as 50ng of genomic DNA input. An optimized dye-labeled nucleotide mix with new dNTP linkers, novel application-specific Alexa Fluor 3 and 5 dyes, and improved buffer chemistry reduce labeling variation and increase signal to noise on arrays. Excitation and emission spectra of Alexa Fluor 3 and 5 dyes are suited for conventional two color scanners with no need to change settings. New master mix formulation streamlines reaction set-ups, increasing consistency. Complete kits are batch tested to assure quality in performance from lot to lot.
Contents and Storage:
The BioPrime™ Total Genomic Labeling Module contains Alexa Fluor 3 and Alexa Fluor 5 2x reaction mix, Exo-Klenow fragment (40 U/μL), 5mM EDTA, TE Buffer, and control DNA (Salmon Sperm). Available in a direct labeling format.

Array CGH, ChIP-on-Chip, Chromatin Biology, DNA and RNA Purification and Analysis, DNA Labeling, Gene Expression Analysis and Genotyping, Genotyping and Genomic Profiling, Nucleic Acid Labeling and Oligo Synthesis, RNAi, Epigenetics and Non-Coding RNA Research