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Invitrogen™ Bacterial Alkaline Phosphatase

Product Code. 10644033
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Quantity:
2500 U
Unit Size:
Each
This item is not returnable. View return policy

For Research Use Only. All usage must comply with product instructions.

Product Code. 10644033

Brand: Invitrogen™ 18011015

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This item is not returnable. View return policy

For Research Use Only. All usage must comply with product instructions.

Description

Includes

A vial of 10X dephosphorylation buffer [100mM Tris-HCl (pH 8.0)].

Removes 3' and 5' phosphates from DNA and RNA

BAP is active at 65°C for at least 1 hr. and is inactivated by phenol extraction

  • Source: Purified from E. coli C90
  • Performance and Quality Testing: Endodeoxyribonuclease, exodeoxyribonuclease, and ribonuclease assays; dephosphorylation efficiency determined
  • Unit Definition: One unit hydrolyzes 1nmol of ATP in 30 min. at 37°C
  • Unit Reaction Conditions: 10mM Tris-HCl (pH 8.0), 1.5mM [γ-32P]ATP, and enzyme in 50μL for 30 min. at 37°C

Dephosphorylation of 5'-phosphorylated termini of vector DNA to prevent self-ligation; Dephosphorylation of 5' termini of nucleic acids prior to forward reaction with kinase; Cloning; Restriction Enzyme Cloning

Order Info

Shipping Conditions: Wet ice

TRUSTED_SUSTAINABILITY

Specifications

Content And Storage Bacterial Alkaline Phosphatase is supplied with a vial of 10X dephosphorylation buffer [100 mM Tris-HCl (pH 8.0)]. Store at -20°C.
Shipping Condition Wet Ice
Enzyme BAP
Quantity 2500 U
Product Type Alkaline Phosphatase

For Research Use Only. Not for use in diagnostic procedures.

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