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Invitrogen™ AccuPrime™ Pfx SuperMix

Product Code. 10043322
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Quantity:
200 rxns
Unit Size:
Each
This item is not returnable. View return policy

For Research Use Only. All usage must comply with product instructions.

Product Code. 10043322

Brand: Invitrogen™ 12344040

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580.80 EUR valid until 2025-12-19
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This item is not returnable. View return policy

For Research Use Only. All usage must comply with product instructions.

Description

Includes

AccuPrime Pfx SuperMix is supplied at 1.1X concentration to allow approximately 10% of the final reaction volume to be used for the addition of primer and template solutions. Reagents sufficient for 200 amplification reactions of 25μL each (4 x 1.125mL) are provided. AccuPrime Pfx SuperMix Components: 22U/mL Thermococcus species KOD thermostable polymerase complexed with anti-KOD antibodies, 66mM Tris-SO4 (pH 8.4), 30.8mM (NH4)2SO4, 11mM KCl, 1.1mM MgSO4, 330μM dNTPs, AccuPrime proteins, stabilizers.

Requires

Template and primers

AccuPrime Pfx SuperMix is a ready-to-use mixture of DNA polymerase, accessory proteins, salts, magnesium, and dNTPs for efficient PCR amplification.

Invitrogen AccuPrime Pfx SuperMix provides reagents for high-fidelity, high-specificity amplification of DNA fragments. High fidelity is provided by a proprietary enzyme preparation containing recombinant DNA polymerase from Thermococcus species KOD with proofreading activity. This ready-to-use mixture of DNA polymerase, accessory proteins, salts, magnesium, and dNTPs is designed for efficient PCR amplification.

Features

  • >26-times higher fidelity than with Taq DNA polymerase
  • Reduced set-up time
  • Produces products with blunt ends

Applications

  • High-fidelity PCR
  • Amplification of fragments up to 15 kb
  • Cloning & mutagenesis

Notes

  • Thermostable AccuPrime Pfx SuperMix proteins enhance primer-template hybridization during every cycle, increasing specificity and yield.

Order Info

Approved for shipment on Wet or Dry Ice

TRUSTED_SUSTAINABILITY

Specifications

Concentration 1.1X
Content And Storage AccuPrime Pfx SuperMix (4 × 1.125 mL)

Store at –20°C in a non-frost-free freezer.
Detection Method Primer-probe
GC-Rich PCR Performance Low
Polymerase AccuPrime Pfx DNA Polymerase
Reaction Speed Standard
Quantity 200 rxns
Shipping Condition Wet or Dry Ice
For Use With (Application) Hot-start PCR, High-fidelity PCR
Fidelity (vs. Taq) 26X
Hot Start Built-In Hot Start
No. of Reactions 200 Reactions
Overhang Blunt
Reaction Format SuperMix or Master Mix
Size (Final Product) 15 kb or less
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For Research Use Only. Not for use in diagnostic procedures.

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