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  7. Invitrogen™ AccuPrime™ Pfx DNA Polymerase 

Invitrogen™ AccuPrime™ Pfx DNA Polymerase

Product Code. 10472482
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No. of Reactions:
1000 Reactions
200 Reactions
Unit Size:
Each
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For Research Use Only. All usage must comply with product instructions.

Product Code. 10472482

Brand: Invitrogen™ 12344024

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475.20 EUR valid until 2025-12-19
BEST PRICE on Promo! Use promo code "24110" to get your promotional price.
This item is not returnable. View return policy

For Research Use Only. All usage must comply with product instructions.

Description

Includes

AccuPrime Pfx DNA Polymerase (100μL, at 2.5U/μL) is provided with 10X AccuPrime reaction mix (1mL) that contains dNTPs and a separate vial (1mL) of 50mM MgSO4. Reagents are provided for 200 amplification reactions of 50μL each.

AccuPrime Pfx DNA Polymerase is ideal for high-fidelity amplification of DNA fragments for downstream applications such as cloning and mutagenesis.

AccuPrime Pfx DNA Polymerase is ideal for high-fidelity amplification of DNA fragments for downstream applications such as cloning and mutagenesis. High fidelity is provided by a proprietary enzyme preparation containing recombinant DNA polymerase from Thermococcus species KOD with proofreading (3'→5' exonuclease) activity. Platinum anti-Pfx DNA polymerase antibodies inhibit polymerase activity, providing hot-start capabilities for improved PCR specificity (see figure). Thermostable AccuPrime accessory proteins enhance specific primer-template hybridization during every cycle of PCR, increasing specificity, yield, and robustness over Platinum Pfx alone.

Benefits of AccuPrime Pfx DNA Polymerase

  • Greater than 26 times higher fidelity than Taq DNA polymerase
  • Amplification of fragments up to 12 kb
  • Minimized PCR optimization

Applications

  • Amplification of DNA from complex genomic, viral, and plasmid templates
  • RT-PCR
  • High-fidelity PCR
  • Hot-start PCR

Notes

  • AccuPrime Pfx DNA Polymerase is especially effective where very high levels of fidelity are required.
  • The 10X AccuPrime Pfx reaction mix contains MgSO4, dNTPs, and thermostable proteins that enhance specific primer-template hybridization during each PCR cycle.

Order Info

Approved for shipment on Wet or Dry Ice

TRUSTED_SUSTAINABILITY

Specifications

Content And Storage • AccuPrime Pfx DNA Polymerase (1 x 100 μL at 2.5 U/μL)
• 10X AccuPrime Pfx Reaction Mix (1 x 1 mL)
• 50 mM MgSO4 (1 x 1 mL)

Store at -20°C.
Detection Method Primer-probe
GC-Rich PCR Performance Low
Polymerase AccuPrime Pfx DNA Polymerase
Reaction Speed Standard
Quantity 200 Reactions
Shipping Condition Wet or Dry Ice
For Use With (Application) Hot-start PCR, High-fidelity PCR
Fidelity (vs. Taq) 26X
Hot Start Built-In Hot Start
No. of Reactions 200 Reactions
Overhang Blunt
Reaction Format Separate Components
Size (Final Product) 12 kb or less
Volume 100 μL
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For Research Use Only. Not for use in diagnostic procedures.

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