missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. PCR Equipment and Supplies
  4. PCR Supplies
  5. Standard PCR Products
  6. Hot Start PCR Reagents and Kits
  7. Invitrogen™ AccuPrime™ GC-Rich DNA Polymerase 

Invitrogen™ AccuPrime™ GC-Rich DNA Polymerase

Product Code. 10033322
Click to view available options
No. of Reactions:
1000 Reactions
200 Reactions
Unit Size:
Each
This item is not returnable. View return policy

For Research Use Only. All usage must comply with product instructions.

Product Code. 10033322

Brand: Invitrogen™ 12337024

Please to purchase this item. Need a web account? Register with us today!

1818.30 EUR valid until 2025-12-31
BEST PRICE on Promo! Use promo code "24357" to get your promotional price.
This item is not returnable. View return policy

For Research Use Only. All usage must comply with product instructions.

Description

Includes

AccuPrime GC-Rich DNA Polymerase (100μL, at 2U/μL) is supplied with two 5X reaction buffers and a separate vial (1mL) of 50mM MgSO4 . Both buffers contain dNTPs, MgSO4 , and AccuPrime accessory proteins. Reaction buffer A (1mL) is for genomic targets. Reaction buffer B (1mL) is for use with cDNA, plasmid, or lambda targets. Sufficient reagents are provided for 200 amplification reactions of 25μL each (at 1 unit of enzyme per reaction).

AccuPrime GC-Rich DNA Polymerase is designed to provide high-yield, high-specificity amplification of difficult-to-amplify templates, such as those with >65% GC content.

AccuPrime GC-Rich DNA Polymerase is designed to provide high-yield, high-specificity amplification of difficult-to-amplify templates, such as those with >65% GC content. The kit offers a choice of buffers for amplifying genomic DNA targets (Buffer A) or non-GC-rich cDNA, plasmid, and lambda-based targets (Buffer B).

AccuPrime GC-Rich DNA Polymerase features

  • High yields for targets up to 5 kb in length
  • AccuPrime accessory proteins for improved PCR specificity
  • Sensitivity down to 5 ng of template DNA

Robust and specific amplifications

The AccuPrime buffers contain thermostable proteins that enhance primer-template hybridization during PCR, increasing the specificity of the reaction. The polymerase itself, from the archaebacterium Pyrolobus fumarius, has a five-fold better processivity than Taq DNA polymerase, and remains active even after 4 hours at 95°C.

Unit definition

One unit of AccuPrime GC-Rich DNA Polymerase is the amount of enzyme required to incorporate 10 nmoles of dNTPs into acid-insoluble material in 30 min. at 74°C.

Order Info

Approved for shipment on Wet or Dry Ice

TRUSTED_SUSTAINABILITY

Especificaciones

Content And Storage • AccuPrime GC-Rich DNA Polymerase (1 x 500 μL at 2 U/μL)
• 5X AccuPrime GC-Rich Buffer A (1 x 5 mL)
• 5X AccuPrime GC-Rich Buffer B (1 x 5 mL)
• 50 mM MgSO4 (1 x 5 mL)

Store at –20°C.
GC-Rich PCR Performance High
Polymerase AccuPrime GC-Rich DNA Polymerase
Reaction Speed Standard
Quantity 1,000 reactions
Shipping Condition Wet or Dry Ice
For Use With (Application) Hot-start PCR
Fidelity (vs. Taq) 2X
Hot Start Built-In Hot Start
No. of Reactions 1000 Reactions
Overhang Blunt
Reaction Format Separate Components
Size (Final Product) 5 kb or less
Volume 500 μL
Mostrar más Mostrar menos

For Research Use Only. Not for use in diagnostic procedures.

Corrección del contenido de un producto

Proporcione sus comentarios sobre el contenido del producto rellenando el siguiente formulario.

Título del producto

Al hacer clic en Enviar, acepta que Fisher Scientific se ponga en contacto con usted en relación con los comentarios que ha proporcionado en este formulario. No compartiremos su información para ningún otro fin. Toda la información de contacto proporcionada se mantendrá de acuerdo con nuestra Política de Privacidad. Política de privacidad.

Gracias por ayudarnos a mejorar nuestra web. Su comentario ha sido enviado