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Invitrogen™ eBioscience™ Intracellular Fixation & Permeabilization Buffer Set

Product Code. 15284728
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15284728 1 kit Each
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Denne vare kan ikke returneres. Se returpolicy
Contains Sodium azide and formaldehyde
Regulatorisk: Dette produkt falder ind under Lov om kemiske stoffer og produkter - lovbekendtgørelse nr. 1755 af 22. december 2006. Hvis det er relevant, skal kunden fremskaffe en licens før levering.
Artikelnummer. 15284728 Leverandør Invitrogen™ Leverandørnr. 88882400

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Denne vare kan ikke returneres. Se returpolicy
Contains Sodium azide and formaldehyde
Regulatorisk: Dette produkt falder ind under Lov om kemiske stoffer og produkter - lovbekendtgørelse nr. 1755 af 22. december 2006. Hvis det er relevant, skal kunden fremskaffe en licens før levering.

Beskrivelse

Indeholder

  • IC Fixation Buffer
  • Permeabilization Buffer (10X)

Designed for use in intracellular staining and flow cytometric analysis and has been specially formulated to reduce non-specific staining of fluorochrome-labelled antibodies and increase fluorescence signal-to-noise ratios.

  • In the first step, live cells can be 'fixed' with the Fixation Buffer, which cross- links proteins
  • The second step, using the Permeabilization Buffer, creates holes in the membrane and allows the intracellular staining antibodies to enter the cell effectively
  • Subsequent washing steps, antibody additions, and incubations after cell permeabilization should be performed using the Permeabilization Buffer
  • Final washing step and cell resuspension prior to running the sample is done using Flow Cytometry Staining Buffer (cat. 00-4222).
  • This kit is commonly used to stain cytoplasmic proteins and proteins in the secretory pathway such as chemokines and cytokines
  • Not recommended for intranuclear staining. Instead, please refer to the Foxp3/Transcription Factor Buffer Set for nuclear staining
  • Before the fixation step, it is recommended to label the live cells using one of the Fixable Viability Dyes and then proceed with fixation
TRUSTED_SUSTAINABILITY

Tekniske data

Product Line eBioscience
Product Type Fixn/Perm Buffer Set
Quantity 1 kit
Solution Type Fixation/Permeabilization Buffer

Sikkerhed og håndtering

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Produktidentifikator
  • eBioscience Intracellular Fixation & Permeabilization Buffer Set
 Signalord
  • Danger
 Farekategori
  • Carcinogenicity Category 1A
  • Serious eye damage/eye irritation Category 1
  • Germ cell mutagenicity Category 2
  • Skin sensitiser Category 1
  • Specific target organ toxicity Category 2
 Faresætning
  • H317-May cause an allergic skin reaction.
  • H318-Causes serious eye damage.
  • H341-Suspected of causing genetic defects.
  • H350-May cause cancer.
  • H371-May cause damage to organs.
 Sikkerhedssætning
  • P201-Obtain special instructions before use.
  • P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
  • P264-Wash hands thoroughly after handling.
  • P270-Do not eat, drink or smoke when using this product.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P302+P352-IF ON SKIN: Wash with plenty of water/soap.
  • P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
  • P308+P313-IF exposed or concerned: Get medical advice/attention.
  • P310-Immediately call a POISON CENTER/doctor/.
  • P332+P313-If skin irritation occurs: Get medical advice/attention.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
 Supplerende oplysninger
  • MIXTURE LIST-Contains : Paraformaldehyde

For Research Use Only. Not for use in diagnostic procedures.

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