Cytiva GFX PCR DNA and Gel Band Purification Kit

Description

illustra GFX PCR DNA and Gel Band Purification Kit is designed for the rapid purification and concentration of PCR products or DNA fragments ranging in size from 50bp to 10kb. This kit can be used to purify DNA from reaction volumes up to 100μl or agarose gel slices up to 900mg. The kit combines a versatile chaotropic buffer with a glass fibre matrix supported in a spin column for the purification of DNA from both solution and agarose gel. Typical recoveries range from 60% to 80% for DNA fragments from agarose gel to as high as 95% for PCR products from solution. DNA purity is exceptional; 99.5% of contaminants are removed. Illustra GFX PCR DNA and Gel Band Purification Kit contains the following components in sufficient quantities: GFX columns, collection tubes, color-coded bottles of capture buffer, wash buffer, and two elution buffers (Tris-HCl and sterile water), and an instruction booklet.

• For the isolation and concentration of DNA fragments (50 bp to 40 kb) from PCR mixtures, DNA-containing agarose gel bands, enzyme-based DNA modifications, and restriction enzyme digests.
• Fast and easy-to-use method with less than 10 minutes hands-on time.
• Flexible 10 to 50μL elution volume for different DNA concentration needs.
• Improved column design with increased maximum volume, holding of up to 800μL liquid volume (950μL without a cap).
• Highly pure DNA is ready for direct use in sequencing, PCR, labeling, restriction enzyme digestion, and cloning.
• Addition of a visual color indicator to the capture buffer ensures optimal pH for maximum DNA binding and recovery.
• Two choices of elution buffer provided to fit downstream applications.
• Color-coded caps for ease of use.