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Invitrogen™ Human IL-12 p40 Matched Antibody Pair

Product Code. 10446243
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10446243 10 x 96 Tests Each
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For Research Use Only. All usage must comply with product instructions.
Product Code. 10446243 Supplier Invitrogen™ Supplier No. CHC1563
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Description

ELISA

The Human Interleukin 12 p40 Antibody Pair Kit comes with pre-matched antibody pairs, standards, and streptavidin-HRP to develop your own enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human IL-12 p40. Buffer reagents needed to complete the ELISA reaction are sold separate as a Buffer Kit for Antibody Pairs (Catalog No. CNB0011). The Assay Buffer included in this Buffer Kit can be used as a blocking reagent for ELISA plates as well as a diluent for ELISA standards and samples, detection antibody, and HRP conjugate. Principle of the method ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

IL12B (IL-12 p40) encodes a subunit of interleukin 12, a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. Interleukin 12 is a disulfide-linked heterodimer composed of the 40 kDa cytokine receptor like subunit encoded by this gene, and a 35 kDa subunit encoded by IL12A. This cytokine is expressed by activated macrophages that serve as an essential inducer of Th1 cells development. This cytokine has been found to be important for sustaining a sufficient number of memory/effector Th1 cells to mediate long-term protection to an intracellular pathogen. Overexpression of this gene was observed in the central nervous system of patients with multiple sclerosis (MS), suggesting a role of this cytokine in the pathogenesis of the disease. The promoter polymorphism of this gene has been reported to be associated with the severity of atopic and non-atopic asthma in children.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number P29460
Assay Range 31.2-2000 pg/mL
Assay Sensitivity <31.2 pg/mL
Conjugate HRP
Gene Accession No. P29459,P29460
Gene Alias CLMF, IL-12A, NFSK, NKSF1, P35, CLMF; CLMF p35; CLMF p40; CLMF2; cytotoxic lymphocyte maturation factor 40 kDa subunit; Il12; IL12 p40; IL-12 p40 subunit; IL-12 subunit p35; IL-12 subunit p40; IL12, subunit p40; IL-12.p40; IL12A; IL-12A; Il12b; IL-12B; Il12p40; IL-12p40; IL-12p40 subunit; IMD28; IMD29; Interleukin; interleukin 12 p40 subunit; interleukin 12 subunit p40; interleukin 12, p40; interleukin 12B; interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40); interleukin-12 beta chain; interleukin-12 beta chain precursor; interleukin-12 p40; interleukin-12 p40 chain; interleukin-12 p40 subunit; interleukin-12 subunit beta; interleukin-12 subunit p40; interleukin-12p40; interleukin-23 p40 subunit; natural killer cell stimulatory factor, 40 kD subunit; NK cell stimulatory factor chain 2; NKSF; NKSF1; NKSF2; p40; RP23-388G23.1CLMF; CLMF p35; CLMF p40; CLMF2; cytotoxic lymphocyte maturation factor 40 kDa subunit; Il12; IL12 p40; IL-12 p40 subunit; IL-12 subunit p35; IL-12 subunit p40; IL12, subunit p40; IL-12.p40; IL12A; IL-12A; Il12b; IL-12B; Il12p40; IL-12p40; IL-12p40 subunit; IMD28; IMD29; Interleukin; interleukin 12 p40 subunit; interleukin 12 subunit p40; interleukin 12, p40; interleukin 12B; interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40); interleukin-12 beta chain; interleukin-12 beta chain precursor; interleukin-12 p40; interleukin-12 p40 chain; interleukin-12 p40 subunit; interleukin-12 subunit beta; interleukin-12 subunit p40; interleukin-12p40; interleukin-23 p40 subunit; natural killer cell stimulatory factor, 40 kD subunit; NK cell stimulatory factor chain 2; NKSF; NKSF1; NKSF2; p40; RP23-388G23.1; CLMF; CLMF2; IL-12B; IMD28; IMD29; NKSF; NKSF2;CLMF; IL-12A; NFSK; NKSF1; P35
Gene Symbols IL12A
Immunoassay Kit Format Matched Antibody Pair
Product Type ELISA, ELISA Kit
Quantity 10 x 96 Tests
Sample Type Plasma, Serum, Supernatant
Gene ID (Entrez) 3592, 3593, 3593
Target Species Human
For Use With (Equipment) Absorbance Microplate Reader, Colorimetric Microplate Reader
Includes Capture (coating) Antibody, Detection Antibody, Standard, Streptavidin-HRP
Content And Storage Antibody pair Kit contains matched, pre-titred and fully optimized Capture (coating) and Detection Antibodies, 3 vials of Recombinant Standard, Streptavidin-HRP concentrate and technical data sheet.
Store at 2-8°C.
Gene Symbol Il12b
Kit Contents Capture (coating) Antibody, Detection Antibody, Standard, Streptavidin-HRP
Label or Dye Biotin
Regulatory Status RUO
Shipping Condition Wet Ice
Storage Requirements 2-8°C
Target IL-12 p40
Test Time 1 hr 30 min
Total Assay Time 24 hr
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