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  5. Invitrogen™ Human HVEM/TNFRSF14 ELISA Kit

Invitrogen™ Human HVEM/TNFRSF14 ELISA Kit

Product Code. 15465153
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Quantity:
5 x 96 Tests
96 Tests
Unit Size:
1 set
Each
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For Research Use Only. All usage must comply with product instructions.

Product Code. 15465153

Brand: Invitrogen™ EHTNFRSF14

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This item is not returnable. View return policy

For Research Use Only. All usage must comply with product instructions.

Description

ELISA

The Human Herpes Virus Entry Mediator (HVEM/TNFSF14) ELISA quantitates Hu HVEM in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu HVEM. Principle of the method The Human HVEM solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

TNFRSF14 is a member of the TNF-receptor superfamily. TNFRSF14 was identified as a cellular mediator of herpes simplex virus (HSV) entry. Binding of HSV viral envelope glycoprotein D (gD) to TNFRSF14 has been shown to be part of the viral entry mechanism. The cytoplasmic region of TNFRSF14 was found to bind to several TRAF family members, which may mediate the signal transduction pathways that activate the immune response. Activation of the signal transduction pathway involving TNFRSF14 in T cells stimulates T cell proliferation and cytokine production, leading to inflammation and enhanced CTL-mediated tumor immunity, suggesting that these proteins may be useful as potential targets for controlling cellular immune responses. Multiple alternatively spliced transcript variants have been described, but the full-length nature of some of these TNFRSF14 variants have not been determined.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number Q92956
Assay Range 32.77 to 8000 pg/mL
Assay Sensitivity 30 pg/mL
Calibration Range 30 pg/mL to 8000 pg/mL
Assay Solid-phase sandwich ELISA
Conjugate HRP
Product Type ELISA
Protein Family Cytokines & Receptors
Sample Type Plasma, Serum, Supernatant
Specificity This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, CNTF, ENA- 78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL- 11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, FGF-4, FGF-6, FGF- 7, G-CSF, GDNF, GM-CSF, IFN-gamma, IGFBP-2, IGFBP-3, IGFBP-4, Leptin (OB), MCP-1, MCP-2, MDC, MIF, MIG, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, SDF-1 alpha, TARC, TGF-beta, TIMP-1, TIMP- 2, TNF-alpha, TNF-beta, TPO, VEGF.
Sufficient For 96 assays
Target Species Human
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 8764
Gene Symbol CD258,HVEML,LIGHT,LTg
Interassay CV <12%
Intraassay CV <10
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 96 Tests
Regulatory Status RUO
Research Discipline Biomarker-Related Pathways
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
Storage Requirements 2°C to 8°C
Synonym RP3-395M20.6, ATAR, CD270, HVEA, HVEM, LIGHTR, TR2
Target TNFRSF14 (HVEM)
Test Time 1 hrs 20 mins
Total Assay Time 4 hrs 45 mins
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