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  5. Invitrogen™ Human Vitronectin (VTN) ELISA Kit

Invitrogen™ Human Vitronectin (VTN) ELISA Kit

Product Code. 15465173
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Quantity:
5 x 96 Tests
96 Tests
Unit Size:
1 set
Each
This item is not returnable. View return policy

For Research Use Only. All usage must comply with product instructions.

Product Code. 15465173

Brand: Invitrogen™ EHVTN

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This item is not returnable. View return policy

For Research Use Only. All usage must comply with product instructions.

Description

ELISA

The Human Vitronectin (VTN) ELISA quantitates Hu VTN in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu VTN. Principle of the method The Human VTN solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

VTN is a member of the pexin family. This protein is found in serum and tissues and promotes cell adhesion and spreading, inhibits the membrane-damaging effect of the terminal cytolytic complement pathway, and binds to several serpin serine protease inhibitors. The protein is a secreted protein and exists in either a single chain form or a clipped, two chain form held together by a disulfide bond.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number P04004
Assay Range 0.819 to 200 ng/mL
Assay Sensitivity 0.8 ng/mL
Calibration Range 0.8 ng/mL to 200 ng/mL
Assay Solid-phase sandwich ELISA
Conjugate HRP
Product Type ELISA
Protein Family Other Proteins
Sample Type Plasma, Serum, Supernatant
Specificity This ELISA kit shows no cross-reactivity with the following cytokines tested: human A2M, ApoA1, ApoC1, ApoC2, ApoC3, ApoE, ApoH, Artemin, BMP-6, Calbindin D, Ck beta 8-1 , CNTF, EMMPRIN, Endostatin, Fibrinogen, FLRG, GITR Ligand, GPBB, Hemoglobin, HTRA2, IL-17 E, IL-1R3, Lactoferrin, Mesothelin, PAPP-A, PDECGF, PDGF Ralpha, Pepsinogen II, Periostin, Persephin, PTH, RAP, SAA, Serpin F1, Tie-1, tPA, TSP-2, Ubiquitin+1, Vitronectin, vWF
Sufficient For 96 assays
Target Species Human
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 7448
Gene Symbol V75,VN,VNT
Interassay CV <12%
Intraassay CV <10
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 96 Tests
Regulatory Status RUO
Research Discipline Cardiovascular Research
Sample Volume Plasma 2 μL, Serum 2 μL, Supernatant 100 μL
Storage Requirements 2°C to 8°C
Synonym V75, VN, VNT
Target Vitronectin
Test Time 1 hrs 20 mins
Total Assay Time 4 hrs 45 mins
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