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Invitrogen™ Human TNF-alpha Recombinant Protein

Product Code. 10056073
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Quantity:
20 μg
Unit Size:
20µg
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For Research Use Only. All usage must comply with product instructions.

Product Code. 10056073

Brand: Invitrogen™ RTNFAI

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This item is not returnable. View return policy

For Research Use Only. All usage must comply with product instructions.

Description

Recombinant Protein

The mature, soluble form of human TNFa was expressed in Pichia pastoris, KM71H. GenBank Accession # P01375, amino acids 77 - 233. For protein verification, the expressed protein was N-terminal sequenced and begins with the following 10 amino acids: VRSSSRTPSD. Purity is >95% by SDS-PAGE analyses. Activity: The biological activity of recombinant human TNFa was measured by its cytolytic effect on mouse L-929 cells in the presence of Actinomycin D. Use: For in vitro applications. A dose-response assay should be performed to determine the optimal concentration for specific experiments. Reconstitution and Dilution: Store lyophilized material at 4°C. Reconstitute in 1 mL of distilled or deionized water. Dilute in a buffer-containing carrier protein, aliquot, and store at or below -70°C, avoiding multiple freeze-thaw cycles. PBS with 1% BSA or a neutral media containing <10% FCS are suitable buffers.

TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number P01375
For Use With (Application) Bioactivity
Formulation PBS with no preservative
Gene ID (Entrez) 7124
Molecular Weight (g/mol) 17.4 kDa
Name Human TNF-alpha
Purification Method Purified
Quantity 20 μg
Source Yeast
Storage Requirements 4°C
Regulatory Status RUO
Gene Alias APC1 protein; Cachectin; C-domain 1; C-domain 2; cTNF; DADB-70P7.1; DIF; ICD1; ICD2; Intracellular domain 1; Intracellular domain 2; N-terminal fragment; NTF; RATTNF; Tnf; TNF alpha; TNF superfamily; TNF α; TNF, macrophage-derived; TNF, monocyte-derived; TNFA; TNF-a; TNFalpha; TNF-alpha; Tnfsf1a; TNFSF2; TNFα; TNLG1F; Tumor necrosis factor; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; tumor necrosis factor alpha (cachetin); tumor necrosis factor alpha precursor; tumor necrosis factor ligand 1F; tumor necrosis factor ligand superfamily member 2; Tumor necrosis factor, membrane form; Tumor necrosis factor, soluble form; tumor necrosis factor-alpha; tumor necrosis factor-alpha precursor; tumor-necrosis factor; tumour necrosis factor
Common Name TNF alpha
Gene Symbol Tnf
Biological Activity Determined by the cytolytic effect on mouse L-929 cells in the presence of Actinomycin D.
Product Type Protein
Conjugate Unconjugated
Species Yeast
Recombinant Recombinant
Sequence Human TNF-alpha, amino acids 77-233 (Accession # P01375)
Content And Storage 4°C
Activity Determined by the cytolytic effect on mouse L-929 cells in the presence of Actinomycin D.
Expression System P. pastoris
Form Lyophilized
Protein Subtype Recombinant
Purity or Quality Grade >95% by SDS-PAGE
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