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Gibco™ Human SPARC Recombinant Protein, PeproTech®

Product Code. 17870133
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17870133 100 μg 100µg
17880013 10 μg 10µg
17860873 1 mg 1mg
17840683 250 μg 250µg
17879922 500 μg 500µg
17811273 50 μg 50µg
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Product Code. 17870133 Supplier Gibco™ Supplier No. 12036100UG

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Description

Recombinant Protein

Recombinant Human SPARC/Osteonectin is a glycoprotein containing 286 amino acids that migrates at an apparent MW of 43.7 kDa by SDS-PAGE analysis due to the effect of glycosylation. The calculated molecular weight of Recombinant Human SPARC/Osteonectin is 32.7 kDa. This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

SPARC gene encodes a cysteine-rich acidic matrix-associated protein. The encoded protein is required for the collagen in bone to become calcified but is also involved in extracellular matrix synthesis and promotion of changes to cell shape. The gene product has been associated with tumor suppression but has also been correlated with metastasis based on changes to cell shape which can promote tumor cell invasion. Three transcript variants encoding different isoforms have been found for this gene.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number P09486
For Use With (Application) Functional Assay
Formulation protein with no preservative
Gene ID (Entrez) 6678
Molecular Weight (g/mol) 32.7 kDa
Name Human SPARC
Quantity 100 μg
Source CHO cells
Regulatory Status RUO
Endotoxin Concentration <1 EU/ μg
Gene Alias Basement membrane protein 40; basement-membrane protein 40; BM40; BM-40; Cysteine rich protein; OI17; ON; Osteonectin; osteonectin precursor; Secreted acidic cystein rich glycoprotein; secreted acidic cysteine rich glycoprotein; Secreted acidic cystein-rich glycoprotein (osteonectin); secreted protein acidic and cysteine rich; secreted protein acidic and rich in cysteine; Secreted protein acidic and rich in cysteine (SPARC); secreted protein, acidic, cysteine-rich (osteonectin); secreted protein, acidic, cysteine-rich [osteonectin]; SPARC
Common Name SPARC
Gene Symbol SPARC
Biological Activity Determined by its ability to inhibit alkaline phosphatase activity in differentiating MC3T3 cells, with an IC50 of 0.5-0.7 ug/ml
Conjugate Unconjugated
Recombinant Recombinant
Sequence APQQEALPDE TEVVEETVAE VTEVSVGANP VQVEVGEFDD GAEETEEEVV AENPCQNHHC KHGKVCELDE NNTPMCVCQD PTSCPAPIGE FEKVCSNDNK TFDSSCHFFA TKCTLEGTKK GHKLHLDYIG PCKYIPPCLD SELTEFPLRM RDWLKNVLVT LYERDEDNNL LTEKQKLRVK KIHENEKRLE AGDHPVELLA RDFEKNYNMY IFPVHWQFGQ LDQHPIDGYL SHTELAPLRA PLIPMEHCTT RFFETCDLDN DKYIALDEWA GCFGIKQKDI DKDLVI
Content And Storage -20°C
Expression System CHO cells
Form Lyophilized
Purity or Quality Grade ≥ 98% by SDS-PAGE gel and HPLC analyses.
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