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Invitrogen™ Human CD25/IL-2R (Soluble) Instant ELISA™ Kit

Product Code. 15551677
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128 Tests
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128 tests
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Product Code. 15551677 Supplier Invitrogen™ Supplier No. BMS212INST

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Description

ELISA

The Human Soluble CD25/Interleukin 2 Receptor(Hu sCD25/IL-2R) ELISA quantitates Hu sCD25/IL-2R in human serum, plasma, urine or cell culture supernatants or other body fluids. The assay will exclusively recognize both natural and recombinant Hu sCD25/IL-2R. Principle of the method The Human sCD25/IL-2R solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

CD25 (IL2 receptor alpha chain/IL2RA) is a cytokine that plays a role in the proliferation of T and B lymphocytes. The receptor of this cytokine (IL2RA) is a heterotrimeric protein complex with a gamma chain also shared by interleukin 4 (IL4) and interleukin 7 (IL7). IL2RA, IL2R beta chain (IL2RB), and the IL2R gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric IL2RA chains result in low-affinity receptor, while homodimeric IL2RB chains produce a medium-affinity receptor. The expression of IL2 in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. IL2 is primarily produced by mature T cells. IL2 plays an important role as a growth factor, differentiation factor, and regulator of cell death. IL-2 stimulates the proliferation of B cells, augments natural killer cell activity, and inhibits granulocyte macrophage colony formation. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests a role in the immune response to antigenic stimuli. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number P01589
Assay Range 0.31 to 20.0 ng/mL
Assay Sensitivity 0.21 ng/mL
Conjugate HRP
Product Type ELISA
Sample Type Plasma, Serum, Supernatant, Urine
Target Species Human
For Use With (Application) ELISA
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 3559
Gene Symbol CD25,IDDM10,IL2R,IMD41,TCGFR,p55
Interassay CV 10%
Intraassay CV 7.7
Kit Contents Pre-coated 96 well Microplate, Detection Antibody, Anti-IgG-HRP, Sample Diluent, lyophilized, Standard (8-well strips x 4), Wash Buffer Concentrate, Substrate Solution, Sample Diluent, Stop Solution, Lysis Buffer 10x, Adhesive Plate Covers
Label or Dye HRP
Quantity 128 Tests
Regulatory Status RUO
Sample Volume Plasma 10 μL, Serum 10 μL, Supernatant 10 μL, Urine 10 μL
Storage Requirements -20°C
Synonym CD25 antigen, TAC, Interleukin-2 receptor alpha
Target CD25
Test Time 15 mins
Total Assay Time 3 hrs 10 mins
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