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Invitrogen™ Human MRPS25 (aa 113-173) Control Fragment Recombinant Protein

Product Code. 30210441
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Quantity:
100 μL
Unit Size:
100µL
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30210441 100 μL 100µL
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Product Code. 30210441 Supplier Invitrogen™ Supplier No. RP100665

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Description

Recombinant Protein

Highest antigen sequence indentity to the following orthologs: Mouse (80%), Rat (80%). This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-60386 (PA5-60386. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein. A pseudogene corresponding to this gene is found on chromosome 4.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number P82663
Concentration ≥5.0 mg/mL
For Use With (Application) Blocking Assay, Control
Formulation 1 M urea, PBS with no preservative; pH 7.4
Gene ID (Entrez) 64432
Name Human MRPS25 (aa 113-173) Control Fragment
Quantity 100 μL
Regulatory Status RUO
Gene Alias 2810429N01Rik; 28 S ribosomal protein S25, mitochondrial; mitochondrial 28 S ribosomal protein S25; mitochondrial ribosomal protein S25; Mitochondrial small ribosomal subunit protein mS25; MRPS25; MRP-S25; RPMS25; rt25; S25mt; Shd10; Unknown (protein for MGC:134542)
Common Name MRPS25
Gene Symbol MRPS25
Conjugate Unconjugated
Species Human
Recombinant Recombinant
Protein Tag His-ABP-tag
Sequence LREEEEEKKQLSHPANFGPRKYCLRECICEVEGQVPCPSLVPLPKEMRGKYKAALKADAQD
Content And Storage -20°C, Avoid Freeze/Thaw Cycles
Expression System E. coli
Form Liquid
Purity or Quality Grade >80% by SDS-PAGE and Coomassie blue staining
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