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  5. Invitrogen™ Human Legumain ELISA Kit

Invitrogen™ Human Legumain ELISA Kit

Product Code. 17135069
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5 x 96 Tests
96 Tests
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17135069 5 x 96 Tests Each
17154593 96 Tests Pack of 96
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For Research Use Only. All usage must comply with product instructions.
Product Code. 17135069 Supplier Invitrogen™ Supplier No. EH299RBX5

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This item is not returnable. View return policy

For Research Use Only. All usage must comply with product instructions.

Description

ELISA

Human Legumain quantitates human Legumain in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human Legumain.

This gene encodes a cysteine protease that has a strict specificity for hydrolysis of asparaginyl bonds. This enzyme may be involved in the processing of bacterial peptides and endogenous proteins for MHC class II presentation in the lysosomal/endosomal systems. Enzyme activation is triggered by acidic pH and appears to be autocatalytic. Protein expression occurs after monocytes differentiate into dendritic cells. A fully mature, active enzyme is produced following lipopolysaccharide expression in mature dendritic cells. Overexpression of this gene may be associated with the majority of solid tumor types. This gene has a pseudogene on chromosome 13. Several alternatively spliced transcript variants have been described, but the biological validity of only two has been determined.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number AEP; AI746452; Asparaginyl endopeptidase; AU022324; cysteine protease 1; legumain; LGMN; LGMN1; preprolegumain; protease, cysteine 1; protease, cysteine, 1; protease, cysteine, 1 (legumain); PRSC1; PRSC1EC 3.4.22.34
Assay Range 28.7 to 7,000 pg/mL
Assay Sensitivity 28.7 pg/mL
Conjugate HRP
Product Type ELISA
Sample Type Plasma, Serum, Supernatant
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 5641
Gene Symbol LGMN
Interassay CV <12%
Intraassay CV <10
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 5 x 96 Tests
Regulatory Status RUO
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Storage Requirements 2°C to 8°C
Target LGMN
Test Time 1 hrs 20 mins
Total Assay Time 4 hrs 45 mins
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