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Proteintech™ Human INS ELISA Kit
Solid phase sandwich ELISA used to detect and quantify protein levels of endogenous INS
Brand: Proteintech™ KE00045-96T
Additional Details : Weight : 0.15000kg
Description
- Recognizes human INS
- Insulin is a peptide hormone, produced by beta cells of pancreas and is central to regulating carbohydrates and fat metabolism in the body
- Participates in glucose utilization, protein synthesis and formation and storage of neutral lipids
- Insulin synthesizes as precursor molecule, proinsulin, which is processed prior to secretion
- A and B peptides are joined together by a disulfide bond to form insulin, while central portion of precursor molecule is cleaved and released as C-peptide
- Defects in insulin results in Type 1 diabetes mellitus
Process:
- Antibody specific for INS pre-coated onto microwells
- INS protein in samples captured by the coated antibody after incubation
- After extensive washing, another antibody of biotinylated specific for INS is added to detect captured INS protein
- For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent
- Solution containing sulfuric acid used to stop color development and color intensity
- Proportional to quantity of bound protein, measurable at 450 nm with correction wavelength set at 630 nm
Specifications
| NP_000198 | |
| 1.3 pmol/L | |
| Sandwich ELISA, Double Antibody | |
| Plasma, Serum | |
| Human | |
| Human | |
| <10% | |
| Blue ice | |
| 4.5 hr. |
| 7.8 to 500 pmol/L | |
| ISO 13485 | |
| Coated ELISA Kit | |
| DAB | |
| 3630 | |
| Solid | |
| <10% | |
| -20°C to +4°C | |
| P01308 |