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Invitrogen™ IL-6 Human ELISA Kit, High Sensitivity

Product Code. 15521687
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96 Tests
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96 tests
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Product Code. 15521687 Supplier Invitrogen™ Supplier No. BMS213HS

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Description

Includes

  • Aluminum pouch(es) with a Microwell Plate coated with monoclonal antibody to human IL-6
  • Biotin-Conjugate anti-human IL-6 monoclonal antibody
  • Streptavidin-HRP
  • Human IL-6 Standard lyophilized, 200pg/mL upon reconstitution
  • Control high, lyophilized
  • Control low, lyophilized
  • Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  • Sample Diluent
  • Amplification Diluent Concentrate (2x)
  • Amplification Reagent I
  • Amplification Reagent II
  • Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
  • Substrate Solution (tetramethyl-benzidine)
  • Stop Solution (1M Phosphoric acid)
  • Adhesive Films

High Sensitivity Sandwich ELISA Kit

The Human High Sensitivity Interleukin 6 (HS IL-6) ELISA quantitates Hu HS IL-6 in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu HS IL-6. Principle of the method The Human HS IL-6 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A special amplification reagent step is added to achieve the high sensitivity capability. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Interleukin 6 (IL-6) is a multifunctional 26 kD protein originally discovered in the medium of RNA-stimulated fibroblastoid cells. IL-6 appears to be directly involved in the responses that occur after infection and cellular injury, and it may prove to be important as IL-1 and TNF-a in regulating the acute phase response. IL-6 is reported to be produced by fibroblasts, activated T cells, activated monocytes or macrophages and endothelial cells. IL-6 acts upon a variety of cells including myeloid progenitor cells, T cells, B cells, and hepatocytes. In addition, IL-6 appears to interact with IL-2 in the proliferation of T lymphocytes. IL-6 potentiates the proliferative effect of IL-3 on multipotential hematopoietic progenitors. IL-6 plays a critical role in B-cell differentiation to plasma cells and is a potent growth factor for plasmacytoma and myeloma. IL-6 is a very useful culture supplement for the generation of a high number of antibody-producing hybridomas. Primarily produced at sites of acute and chronic inflammation, IL-6 is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of IL-6 is implicated in a wide variety of inflammation-associated disease states including diabetes mellitus and systemic juvenile rheumatoid arthritis.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number P05231
Assay Range 0.08-5.0 pg/mL
Assay Sensitivity 0.03 pg/mL
Conjugate Biotin
Product Type ELISA Kit
Sample Type Plasma, Serum, Supernatant
Target Species Human
For Use With (Application) ELISA
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 3569
Gene Symbol BSF-2,BSF2,CDF,HGF,HSF,IFN-beta-2,IFNB2,IL-6
Interassay CV 6%
Intraassay CV 4.9%
Kit Contents Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, Amplification Diluent Concentrate, Amplification Reagent I, Amplification Reagent II, SAV-HRP, Controls, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 96 Tests
Regulatory Status RUO
Sample Volume Plasma, 50 μL; Serum, 50 μL; Supernatant, 50 μL
Storage Requirements 2°C to 8°C
Synonym Interferon-beta 2, B-cell stimulatory factor 2,HGF
Target Human
Test Time 1 hr. 20 min.
Total Assay Time 4 hr.
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