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  5. Invitrogen™ Human IL-6 ELISA Kit

Invitrogen™ Human IL-6 ELISA Kit

Product Code. 10390964
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Quantity:
2 Plate Kit
5 Plate Kit
96 Tests
Unit Size:
1 set
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For Research Use Only. All usage must comply with product instructions.

Product Code. 10390964

Brand: Invitrogen™ EH2IL6

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560.88 EUR valid until 2025-12-31
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This item is not returnable. View return policy

For Research Use Only. All usage must comply with product instructions.

Description

ELISA

The Human Interleukin-6 (Hu IL-6) ELISA quantitates Hu IL-6 in human serum, plasma, urine, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IL-6. Principle of the method The Human IL-6 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

IL-6 is a gene that encodes a cytokine that plays a role in inflammation and the maturation of B cells. The protein produced by this gene is an endogenous pyrogen that can induce fever in people with autoimmune diseases or infections. The gene is primarily expressed at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Elevated levels of the encoded protein have been found in virus infections, including COVID-19 (disease caused by SARS-CoV-2). Diseases associated with IL6 include Kaposi Sarcoma and Rheumatoid Arthritis, Systemic Juvenile.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number P05231
Assay Range 10.24 to 400 pg/mL
Assay Sensitivity <1 pg/mL
Description Human IL-6 ELISA Kit
Conjugate HRP
Immunoassay Kit Format Colorimetric ELISA (TMB substrate) with Plates
Product Type ELISA
Sample Type Plasma, Serum, Supernatant, Urine
Specificity No significant cross-reaction with with human IL-1 A, IL-1ß, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-10, IL-12, TNFa, IFNg, IFNa, GM-CSF, or mouse IL-6
Sufficient For 96 microplate wells
Target Species Human
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 3569
Gene Symbol BSF-2,BSF2,CDF,HGF,HSF,IFN-beta-2,IFNB2,IL-6
Interassay CV <10%
Intraassay CV <10
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Measurement Absorbance at 450 nm minus absorbance (at 550 nm)
Precision Intra-assay CV <10%; inter-assay CV <10%
Quantity 96 Tests
Regulatory Status RUO
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 50 μL, Urine 50 μL
Storage Requirements 2°C to 8°C
Target IL-6
Test Time 1 hrs 20 mins
Total Assay Time 3 hrs
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Safety and Handling

compliance-icons
Product Identifier
  • Human IL-6 ELISA Kit
 Signal Word
  • Warning
 Hazard Category
  • Skin sensitiser Category 1
 Hazard Statement
  • H317-May cause an allergic skin reaction.
 Precautionary Statement
  • P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
  • P272-Contaminated work clothing should not be allowed out of the workplace.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P302+P352-IF ON SKIN: Wash with plenty of water/soap.
  • P333+P313-If skin irritation or rash occurs: Get medical advice/attention.
  • P362+P364-Take off contaminated clothing and wash it before reuse.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
 Supplemental information
  • MIXTURE LIST-Contains : 2-methyl-3(2H)-isothiazolone
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