missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Immunoassay Reagents and Kits
  4. Life Science Research ELISA Kits
  5. Invitrogen™ Human IL-18 ELISA Kit

Invitrogen™ Human IL-18 ELISA Kit

Product Code. 15591847
Click to view available options
Quantity:
10 x 96 Tests
96 Tests
Unit Size:
96 tests
Pack of 10
This item is not returnable. Se returpolicy

Produktkod. 15591847

missing translation for 'mfr': Invitrogen™ BMS2672

Please to purchase this item. Need a web account? Register with us today!

527.44 EUR valid until 2025-12-31
BEST PRICE on Promo! Use promo code "24080" to get your promotional price.
Denna artikel kan inte returneras. Se returpolicy

Beskrivning

Innehåller

Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human IL-18
Biotin-Conjugate anti-human IL-18 monoclonal antibody
Streptavidin-HRP
Human IL-18 Standard lyophilized, 10ng/mL upon reconstitution
Control high, lyophilized
Control low, lyophilized
Sample Diluent
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Red-Dye
Adhesive Films

ELISA

The Human Interleukin-18 (Hu IL-18) ELISA quantitates Hu IL-18 in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IL-18. Principle of the method The Human IL-18 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

The protein encoded by this gene is a proinflammatory cytokine. This cytokine can induce the IFN-gamma production of T cells. The combination of this cytokine and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL-18 binding protein can specifically interact with this cytokine, and thus negatively regulate its biological activity.
TRUSTED_SUSTAINABILITY

Specifikationer

Accession Number Q14116
Assay Range 78 to 5,000 pg/mL
Assay Sensitivity 9.0 pg/mL
Conjugate HRP
Product Type ELISA
Sample Type Plasma, Serum, Supernatant
Target Species Human
For Use With (Application) ELISA
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 3606
Gene Symbol IGIF,IL-18,IL-1 g,IL1F4
Interassay CV 8.1%
Intraassay CV 6.5
Kit Contents Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Controls, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 96 Tests
Regulatory Status RUO
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Storage Requirements 2°C to 8°C
Synonym Interleukin-18, Interferon-gamma-inducing factor
Target IL-18
Test Time 1 hrs 20 mins
Total Assay Time 3 hrs 10 mins
Visa mer Visa mindre
Korrigering av produktinnehåll

Din input är viktig för oss. Fyll i det här formuläret för att ge feedback relaterad till innehållet på denna produkt.

Produkttitel

Genom att klicka på Skicka bekräftar du att du kan bli kontaktad av Fisher Scientific angående feedbacken du har lämnat i detta formulär. Vi kommer inte att dela din information för andra ändamål. All kontaktinformation som tillhandahålls ska också underhållas i enlighet med vår Sekretesspolicy.

Tack! Din feedback har skickats. Fisher Scientific arbetar alltid för att förbättra vårt innehåll åt dig. Vi uppskattar din feedback.