missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Immunoassay Reagents and Kits
  4. Life Science Research ELISA Kits
  5. 16861476

Proteintech™ Human IDH1 ELISA Kit

Product Code. 16861476 Shop All Proteintech Products
Change view
Click to view available options
Target Species:
Human
Unit Size:
96 tests
Product Code. Target Species unitSize
16861476 Human 96 tests
1 options
This item is not returnable. View return policy

Product Code. 16861476

Brand: Proteintech™ KE0006096T

Please to purchase this item. Need a web account? Register with us today!

This item is not returnable. View return policy

Beskrivelse

Solid phase sandwich ELISA used to detect and quantify protein levels of endogenous IDH1

  • Recognizes human IDH1
  • IDH1 (PICD, IDP) belongs to isocitrate and isopropylmalate dehydrogenases family
    • IDH1 may be tumor suppressor, when mutationally inactivated, contributes to tumorigenesis in part through induction HIF-1 pathway
    • Common feature of major subset of primary human brain cancers
    • IDH1 mutation heterozygotic, functions as dimer

Process:

  • Antibody specific for IDH1 pre-coated onto microwells
  • IDH1 protein in samples captured by the coated antibody after incubation
  • After extensive washing, another antibody specific for IDH1 is added to detect captured IDH1 protein
  • For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent
  • Solution containing sulfuric acid used to stop color development and color intensity
    • Proportional to quantity of bound protein, measurable at 450 nm with correction wavelength set at 630 nm

Tekniske data

Accession Number NP_001269315
Assay Range 62.5 to 4000 pg/mL
Assay Sensitivity 3.3 pg/mL
Certifications/Compliance ISO 13485
Detection Method Sandwich ELISA, Double Antibody
Immunoassay Kit Format Coated ELISA Kit
Sample Type Plasma, Serum
Substrate DAB
Target Species Human
Gene ID (Entrez) 3417
Host Species Human
Immunoassay Kit Grade Solid
Interassay CV <10%
Intraassay CV <10%
Shipping Condition Blue ice
Storage Requirements -20°C to +4°C
Total Assay Time 3.5 hr.
UniProt ID Q6FHQ6
Vis mere Vis mindre

Sikkerhed og håndtering

compliance-icons compliance-icons
Produktidentifikator
  • 96TEST Human IDH1 ELISA Kit
 Signalord
  • Warning
 Farekategori
  • Serious eye damage/eye irritation Category 1
  • Serious eye damage/eye irritation Category 2
  • Skin corrosion/irritation Category 2
  • Specific target organ toxicity Category 3
 Faresætning
  • H302-Harmful if swallowed.
  • H315-Causes skin irritation.
  • H318-Causes serious eye damage.
  • H319-Causes serious eye irritation.
  • H332-Harmful if inhaled.
  • H335-May cause respiratory irritation.
 Sikkerhedssætning
  • P201-Obtain special instructions before use.
  • P202-Do not handle until all safety precautions have been read and understood.
  • P234-Keep only in original container.
  • P264-Wash skin thoroughly after handling.
  • P270-Do not eat, drink or smoke when using this product.
  • P273-Avoid release to the environment.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
  • P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
  • P302+P352-IF ON SKIN: Wash with plenty of water/soap.
  • P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
  • P308+P313-IF exposed or concerned: Get medical advice/attention.
  • P330-Rinse mouth.
  • P362-Take off contaminated clothing.
  • P405-Store locked up.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
 Supplerende oplysninger
  • MIXTURE LIST-Contains : Sulphuric Acid, Tetramethylbenzidine
Produkttitel
Vælg et problem

Ved at klikke på Send, anerkender du, at du kan blive kontaktet af Fisher Scientific med hensyn til den feedback, du har givet i denne formular. Vi deler ikke dine oplysninger til andre formål. Alle angivne kontaktoplysninger skal også vedligeholdes i overensstemmelse med vores Privatlivspolitik.