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Proteintech™ Human Galectin-3 ELISA Kit
Solid phase sandwich ELISA used to detect and quantify protein levels of endogenous human Galectin-3
Brand: Proteintech™ KE00126-96T
Additional Details : Weight : 0.15000kg
Description
- Recognizes human Galectin-3
- Galectins are a family of animal lectins defined by shared characteristic amino acid sequences and affinity for β-galactose-containing oligosaccharides
- Galectin-3 is a 31 kDa member of β-galactoside-binding proteins with one carbohydrate recognition domain and a proline and glycine rich N-terminal domain through which is able to form oligomers
- Widely expressed in many normal tissues and tumors
- Found intracellularly in nucleus and cytoplasm or secreted outside of cell, present on cell surface or in extracellular space
- Involved in biological processes (cell growth, adhesion, differentiation, apopotosis, angiogenesis, immune response, neoplastic transformation, metastasis)
- Elevated serum Galectin-3 levels reported in patients with breast, gastrointestinal, lung or ovarian cancer, melanoma and non-Hodgkin's lymphoma
Process:
- Antibody specific for human Galectin-3 pre-coated onto microwells
- Human Galectin-3 protein in samples captured by the coated antibody after incubation
- After extensive washing, another antibody of biotinylated specific for human Galectin-3 is added to detect captured human Galectin-3 protein
- For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent
- Solution containing sulfuric acid used to stop color development and color intensity
- Proportional to quantity of bound protein, measurable at 450 nm with correction wavelength set at 630 nm
Specifications
BC001120 | |
0.16 ng/mL | |
Sandwich ELISA, Double Antibody | |
Plasma, Serum | |
Human | |
Human | |
<10% | |
Blue ice | |
4.5 hr. |
0.625 to 40 ng/mL | |
ISO 13485 | |
Coated ELISA Kit | |
DAB | |
3958 | |
Solid | |
<10% | |
-20°C to +4°C | |
P17931 |