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Proteintech™ Human CYB5R3 ELISA Kit

Product Code. 16831486 Shop All Proteintech Products
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Target Species:
Human
Unit Size:
96 tests
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Product Code. Target Species Unit Size
16831486 Human 96 tests
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Product Code. 16831486 Supplier Proteintech™ Supplier No. KE0006996T
 Restricted Item
Estimated Shipment: 13-11-2026
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Description

Solid phase sandwich ELISA used to detect and quantify protein levels of endogenous CYB5R3

  • Recognizes human CYB5R3
  • Kit for testing serum, plasma and cell lysates
  • CYB5R3 (DIA1, B5R) belongs to flavoprotein pyridine nucleotide cytochrome reductase family
    • Two naturally occurring isoforms
      • Isoform 1: anchored to cytoplasmic side of endoplasmic reticulum membrane and mitochrondrion outer membrane
      • Isoform 2: soluble form found in red blood cells

Process:

  • Antibody specific CYB5R3 pre-coated onto microwells
  • CYB5R3 protein in samples captured by the coated antibody after incubation
  • After extensive washing, another antibody specific for CYB5R3 is added to detect captured CYB5R3 protein
  • For signal detection, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent
  • Solution containing sulfuric acid used to stop color development and color intensity
    • Proportional to quantity of bound protein, measurable at 450 nm with correction wavelength set at 630 nm

Specifications

Accession Number NP_000389
Assay Range 31.25 to 2000 pg/mL
Assay Sensitivity 3.5 pg/mL
Certifications/Compliance ISO 13485
Detection Method Sandwich ELISA, Double Antibody
Immunoassay Kit Format Coated ELISA Kit
Sample Type Plasma, Serum
Substrate DAB
Target Species Human
Gene ID (Entrez) 1727
Host Species Human
Immunoassay Kit Grade Solid
Interassay CV <10%
Intraassay CV <10%
Shipping Condition Blue ice
Storage Requirements -20°C to +4°C
Total Assay Time 3.5 hr.
UniProt ID P00387
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Safety and Handling

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Product Identifier
  • 96TEST Human ORM1 ELISA Kit
 Signal Word
  • Warning
 Hazard Category
  • Serious eye damage/eye irritation Category 1
  • Serious eye damage/eye irritation Category 2
  • Skin corrosion/irritation Category 2
  • Specific target organ toxicity Category 3
 Hazard Statement
  • H302-Harmful if swallowed.
  • H315-Causes skin irritation.
  • H318-Causes serious eye damage.
  • H319-Causes serious eye irritation.
  • H332-Harmful if inhaled.
  • H335-May cause respiratory irritation.
 Precautionary Statement
  • P201-Obtain special instructions before use.
  • P202-Do not handle until all safety precautions have been read and understood.
  • P234-Keep only in original container.
  • P264-Wash skin thoroughly after handling.
  • P270-Do not eat, drink or smoke when using this product.
  • P273-Avoid release to the environment.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
  • P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
  • P302+P352-IF ON SKIN: Wash with plenty of water/soap.
  • P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
  • P308+P313-IF exposed or concerned: Get medical advice/attention.
  • P330-Rinse mouth.
  • P362-Take off contaminated clothing.
  • P405-Store locked up.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
 Supplemental information
  • MIXTURE LIST-Contain: Sulphuric Acid, Tetramethylbenzidine
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