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Proteintech™ Human CD147 ELISA Kit
Solid phase sandwich ELISA used to detect and quantify protein levels of endogenous CD147
Brand: Proteintech™ KE00135-96T
Additional Details : Weight : 0.15000kg
Description
- Recognizes human CD147
- CD147 (Basigin, BSG or EMMPRIN) is a transmembrane glycoprotein belonging to immunoglobulin superfamily
- Important in numerous physiological and pathological phenomena
- Involved in reproduction, neural function, inflammation and tumor invasion
- CD147 expressed at high levels at retinal pigment epithelium and neonatal blood-brain barrier on tumor cells and activated T cells
- Can stimulate adjacent fibroblasts to produce matrix metalloproteinases
- Effective marker to predict invasion and prognosis in various cancers
- Soluble CD147 in body fluids associated with systemic sclerosis, hepatocellular carcinoma, ankylosing spondylitis and coronary diseases
Process:
- Antibody specific for CD147 pre-coated onto microwells
- CD147 protein in samples captured by the coated antibody after incubation
- After extensive washing, another antibody of biotinylated specific for human CD147 is added to detect captured human CD147 protein
- For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent
- Solution containing sulfuric acid used to stop color development and color intensity
- Proportional to quantity of bound protein, measurable at 450 nm with correction wavelength set at 630 nm
Specifications
| BC009040 | |
| 2.1 pg/mL | |
| Sandwich ELISA, Double Antibody | |
| Plasma, Serum | |
| Human | |
| Human | |
| <10% | |
| Blue ice | |
| 4.5 hr. |
| 31.25 to 2000 pg/mL | |
| ISO 13485 | |
| Coated ELISA Kit | |
| DAB | |
| 682 | |
| Solid | |
| <10% | |
| -20°C to +4°C | |
| P35613 |