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Proteintech™ Human CCL5 ELISA Kit

Product Code. 16841506 Shop All Proteintech Products
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Target Species:
Human
Unit Size:
96 tests
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Product Code. Target Species Unit Size
16841506 Human 96 tests
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Product Code. 16841506 Supplier Proteintech™ Supplier No. KE0009396T
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Description

Solid phase sandwich ELISA used to detect and quantify protein levels of endogenous human CCL5

  • Recognizes human CCL5
  • CCL5 (RANTES) belongs to CC chemokine family
    • Strongly expressed in human and murine liver upon injury
    • Produced by cell types: platelets, macrophates, endothelial and stellate cells
    • CCL5 recruits T cells, dendritic cells, eosinophils, NK cells, mast cells and basophils to sites of inflammation by interacting with G-protein-couples receptors (CCR1, CCR3 and CCR5)
      • CCRS5: promote efficient anti-tumor immune responses and associated with cancer progression and metastasis

Process:

  • Antibody specific for human CCL5 pre-coated onto microwells
  • Human CCL5 protein in samples captured by the coated antibody after incubation
  • After extensive washing, another antibody of biotinylated specific for human CCL5 is added to detect captured human CCL5 protein
  • For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent
  • Solution containing sulfuric acid used to stop color development and color intensity
    • Proportional to quantity of bound protein, measurable at 450 nm with correction wavelength set at 630 nm

Specifikationer

Accession Number NP_002976
Assay Range 3.9 to 250 pg/mL
Assay Sensitivity 0.3 pg/mL
Certifications/Compliance ISO 13485
Detection Method Sandwich ELISA, Double Antibody
Immunoassay Kit Format Coated ELISA Kit
Sample Type Plasma, Serum
Substrate DAB
Target Species Human
Gene ID (Entrez) 6352
Host Species Human
Immunoassay Kit Grade Solid
Interassay CV <10%
Intraassay CV <10%
Shipping Condition Blue ice
Storage Requirements -20°C to +4°C
Total Assay Time 4.5 hr.
UniProt ID P13501
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Produktbeteckning
  • 96TEST Human IL-3 ELISA Kit
 Signalord
  • Warning
 Riskkategorier
  • Serious eye damage/eye irritation Category 1
  • Serious eye damage/eye irritation Category 2
  • Skin corrosion/irritation Category 2
  • Specific target organ toxicity Category 3
 Faroangivelser
  • H302-Harmful if swallowed.
  • H315-Causes skin irritation.
  • H318-Causes serious eye damage.
  • H319-Causes serious eye irritation.
  • H332-Harmful if inhaled.
  • H335-May cause respiratory irritation.
 Skyddsangivelse
  • P201-Obtain special instructions before use.
  • P202-Do not handle until all safety precautions have been read and understood.
  • P234-Keep only in original container.
  • P264-Wash skin thoroughly after handling.
  • P270-Do not eat, drink or smoke when using this product.
  • P273-Avoid release to the environment.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
  • P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
  • P302+P352-IF ON SKIN: Wash with plenty of water/soap.
  • P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
  • P308+P313-IF exposed or concerned: Get medical advice/attention.
  • P330-Rinse mouth.
  • P362-Take off contaminated clothing.
  • P405-Store locked up.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
 Kompletterande information
  • MIXTURE LIST-Contain: Sulphuric Acid, Tetramethylbenzidine
Produkttitel
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