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  6. Histone H2AX, p Ser139 Antibody (3F2), Novus Biologicals™

Histone H2AX, p Ser139 Antibody (3F2), Novus Biologicals™

Mouse Monoclonal Antibody has been used in 6 publications

226.00 €

Specifications

Antigen Histone H2AX (p Ser139)
Clone 3F2
Concentration 1 mg/mL
Dilution Western Blot 1 μg/mL, Simple Western 10 μg/mL, Flow Cytometry 1 μg 106 cells, ELISA 1:100 - 1:2000, Immunohistochemistry 1:10 - 1:500, Immunocytochemistry/Immunofluorescence 2 - 4 μg/mL, Immunohistochemistry-Paraffin 1:10 - 1:500
Applications Western Blot
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Novus Biologicals
NB100-74435-0.025mg 		0.025 mg
226.00 €
25µg
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Description

Description

Histone H2AX (p Ser139) Monoclonal antibody specifically detects Histone H2AX (p Ser139) in Human, Mouse, Bovine samples. It is validated for Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin).
Specifications

Specifications

Histone H2AX (p Ser139)
1 mg/mL
Western Blot
Unconjugated
Mouse
Checkpoint signaling, DNA Double Strand Break Repair, DNA Repair, Epigenetics, Mitotic Regulators, Phospho Specific
P16104
3014
This Histone H2AX [p Ser139] Antibody (3F2) was developed against a synthetic peptide sequence surrounding phosphorylated Ser139.
Primary
Store at -20°C. Avoid freeze/thaw cycles.
15 kDa
3F2
Western Blot 1 μg/mL, Simple Western 10 μg/mL, Flow Cytometry 1 μg 106 cells, ELISA 1:100 - 1:2000, Immunohistochemistry 1:10 - 1:500, Immunocytochemistry/Immunofluorescence 2 - 4 μg/mL, Immunohistochemistry-Paraffin 1:10 - 1:500
Monoclonal
Purified
RUO
Human
H2A.X, H2A/X, H2AFX
H2AFX
IgG1 κ
Protein G purified
In Western blot this antibody detects ∼17 kDa protein representing phosphorylated H2AX in gamma irradiated HeLa cell lysate. In immunofluorescence procedures, recognizes phosphorylated H2AX in gamma irradiated HeLa cells. ELISA of phosphorylated H2AX can also be performed. Used in IHC to successfully detect H2A.X pSer140 in postnatal mouse lung section.
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