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  6. Thermo Scientific™ HisPur™ Ni-NTA Superflow Agarose

Thermo Scientific™ HisPur™ Ni-NTA Superflow Agarose

Product Code. 13494189 Shop All Thermo Scientific Products
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Quantity:
10 mL
50 mL
250 mL
1 L
Unit Size:
1 L
10mL
250mL
50mL
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Product Code. 13494189

Brand: Thermo Scientific™ 25216

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2011.75 EUR valid until 2025-12-19
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Description

Perform FPLC purification of poly-histidine-tagged proteins with this nitrilotriacetic acid (NTA) modified Superflow 6 support charged with divalent nickel (Ni+2).

Perform FPLC purification of poly-histidine-tagged proteins with Thermo Scientific™ HisPur Ni-NTA Superflow Agarose, a nitrilotriacetic acid (NTA) modified Superflow 6 support charged with divalent nickel (Ni+2).

Additional Information and Online Ordering

HisPur Ni-NTA Superflow Agarose is a highly crosslinked, durable resin that does not compress at flow rates used for medium- or large-scale FPLC purifications. The resin holds up well to a variety of chemicals and pH values, and is compatible with common clean-in-place procedures. Compared to cobalt and other ligands used for immobilized metal affinity chromatography (IMAC), nickel provides greater capacity for His-tagged protein purification. HisPur Ni-NTA Superflow Agarose exhibits a high dynamic binding capacity across a range of flow rates, making it an excellent choice for larger scale purifications.

Highlights:

  • High capacity – binds greater than 20mg of His-tagged GFP per mL of resin at a linear flow rate of 300cm/hour

  • High purity – provides greater than 80% purity of eluted fractions when used to purify from lysates

  • Versatile – can be used to purify proteins under native and denaturing conditions

  • Robust – highly crosslinked beads tolerate linear flow rates up to 1200cm/hour

  • Compatible – maintains function in a wide variety of chemicals and pH conditions

  • Cost effective – competitively priced resin is stable through multiple cycles of cleaning and reuse

Recommended for:

Medium- to large-scale FPLC purification of polyhistidine tagged proteins

TRUSTED_SUSTAINABILITY

Specifications

Capacity (Metric) 20 mg His-Tagged GFP Binding per mL Resin
Content And Storage Store at 4°C
Form Liquid Suspension
Product Line HisPur
Quantity 250 mL
Target His-Tagged Protein
Type Agarose
Stationary Phase Divalent Nickel, Metal Chelate
Format Bottle
Column Type Affinity, Agarose Resin

Safety and Handling

compliance-icons compliance-icons
Product Identifier
  • HisPur Ni-NTA Superflow Agarose
 Signal Word
  • Warning
 Hazard Category
  • Serious eye damage/eye irritation Category 2
  • Flammable liquid Category 3
 Hazard Statement
  • H226-Flammable liquid and vapour.
  • H319-Causes serious eye irritation.
 Precautionary Statement
  • P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
  • P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
  • P264-Wash hands thoroughly after handling.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
  • P337+P313-If eye irritation persists: Get medical advice/attention.
  • P403+P235-Store in a well-ventilated place. Keep cool.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
 Supplemental information
  • MIXTURE LIST-Contains : ethanol

For Research Use Only. Not for use in diagnostic procedures.

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