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Applied Biosystems™ High-Capacity RNA-to-cDNA™ Kit

The High Capacity RNA-to-cDNA Kit is a streamlined reverse transcription kit designed for optimum performance with TaqMan Gene Expression Master Mix, Power SYBR Green PCR Master Mix, and other PCR enzymes.

299.00 € - 2705.00 €

Specifications

Format Kit
Reaction Speed 30 to 60 min.
Technique Reverse Transcription
Optimal Reaction Temperature 37°C
Reverse Transcriptase MultiScribe
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Products 2
Product Code Brand No. of Reactions Price Quantity & Availability  
Product Code Brand No. of Reactions Price Quantity & Availability  
10704217
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Applied Biosystems™
4387406
50 Reactions
299.00 €
Each
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16271891
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Applied Biosystems™
4388950
500 Reactions
2705.00 €
500 reactions
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Description

Description

The High Capacity RNA-to-cDNA Kit is a streamlined reverse transcription kit designed for optimum performance with TaqMan Gene Expression Master Mix, Power SYBR Green PCR Master Mix, and other PCR enzymes. The components of the two-tube kit work together to provide sensitive and specific reverse transcription across a broad range of template amounts.

Features of the High Capacity RNA-to-cDNA Kit include:
• Fast reaction time (typically 30–60 min)
• Convenient and simple workflow with few pipetting steps
• Reliable reverse transcription of both abundant and limited targets
• Optimized two-step protocol enables multiple PCR reactions from a single RT reaction

Streamline cDNA synthesis while maintaining detection sensitivity
Amplification of a dilution series of the RNA concentration reference standard demonstrates exceptional reverse transcription and PCR efficiency across 11 orders of magnitude. This wide dynamic range allows one set of reaction conditions to detect transcripts from highly active as well as weakly expressed genes. The two-tube formulation reduces reaction time and requires fewer pipetting steps than with the High Capacity cDNA Reverse Transcription Kit, while maintaining high performance.

Specifications

Specifications

Kit
Reverse Transcription
MultiScribe
Dry Ice
First-Strand cDNA
Reverse Transcription
RNA
30 to 60 min.
37°C
Yes
Real Time PCR (qPCR), RT-PCR
Premixed Components
Up to 7 kb
Videos
SDS
Documents

Documents

Product Certifications

For Research Use Only. Not for use in diagnostic procedures.