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Molecular Probes™ Fluoresceinyl Glycine Amide (5-(Aminoacetamido)Fluorescein)
Description
The primary aliphatic amine of 5-(aminoacetamido)fluorescein (fluoresceinyl glycine amide) can be reversibly coupled to aldehydes and ketones to form a Schiff base - which can be reduced to a stable amine derivative by sodium borohydride (NaBH4) or sodium cyanoborohydride (NaCNH3) to form new probes. Carboxylic acids of proteins and other water-soluble biopolymers can be coupled to this molecule in aqueous solution using water-soluble carbodiimides such as EDAC (E2247). The glycine may be the better amine probe for direct carbodiimide-mediated coupling, since it is likely to remain unprotonated at a lower pH than other aliphatic amines.
Specifications
Specifications
| Quantity | 10 mg |
| Product Type | Fluoresceinyl Glycine Amide |
| Content And Storage | Store at room temperature and protect from light. |
| Chemical Reactivity | Carboxylic Acid, Ketone, Aldehyde |
| Shipping Condition | Room Temperature |
| Reactive Moiety | Amine, Primary Amine |
| Label or Dye | FITC (Fluorescein) |
| Label Type | Classic Dyes |
For Research Use Only. Not for use in diagnostic procedures.
Product Title
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