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Invitrogen™ Fluorescein-D22 Coactivator Peptide, 100 , μM
Contains known interaction motifs and labeled with fluorescein. Invitrogen™ Fluorescein-D22 Coactivator Peptide, 100μM is matched to complement the LanthaScreen™ TR-FRET Nuclear Receptor Coregulator Assays to enable primary or secondary screening of nuclear receptor agonists and antagonists.
Brand: Invitrogen™ PV4386
Additional Details : Weight : 0.50000kg
Description
Ligand binding to nuclear receptors causes conformational changes in the receptor, resulting in a cascade of events, including dissociation of repressor proteins, association of coactivator proteins, and assembly of pol II and other transcriptional factors for activation of target genes. TR-FRET based assays can be developed using the LanthaScreen™ panel of fluorescein-labeled coregulator peptides to investigate conformational changes of nuclear receptors upon ligand binding, either by determining the affinity of ligand-bound receptor for different coregulator peptides, or by identifying additional agonists or antagonists via displacement or recruitment of a specific coregulator peptide.
In the LanthaScreen™ TR-FRET RXR beta Coactivator Assay, a terbium (Tb)-labeled anti-GST antibody is used to indirectly label GSTtagged RXR beta protein. An agonist (9-cis Retinoic Acid) added to the receptor which, upon ligand binding, causes a conformational change resulting in recruitment of the Fl-D22 coactivator peptide. The binding of the fluorescent coregulator peptide to RXR beta causes an increase in the TR-FRET emission ratio.
Specifications
| Store in freezer (-5 to -30°C). | |
| Target & Lead Identification & Validation, Nuclear Receptor Biology, Nuclear Receptor Proteins, Biochemical Nuclear Receptor Assays, Nuclear Receptor Coregulator Interaction, Industrial & Applied Science, Pharma & Biopharma | |
| 100 μL | |
| RUO - research use only |