missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Cell Analysis Products
  4. Cell Based Assays
  5. Cell Function Reagents and Kits
  6. Invitrogen™ eBioscience™ Fixable Viability Dye eFluor™ 520

Invitrogen™ eBioscience™ Fixable Viability Dye eFluor™ 520

Product Code. 15590607
Click to view available options
Quantity:
100 tests
500 tests
Unit Size:
100 tests
500 tests
This item is not returnable. View return policy

Product Code. 15590607

Brand: Invitrogen™ 65086718

Please to purchase this item. Need a web account? Register with us today!

This item is not returnable. View return policy

Description

Can be used to irreversibly label dead cells prior to cryopreservation, fixation and/or permeabilization procedures. Unlike 7-AAD and PI, cells labeled with Fixable Viability Dyes can be washed, fixed, permabilized and stained for intracellular antigens without loss of staining intensity.

  • Fixable Viability Dyes may be used to label cells from all species
  • Fixable Viability Dye eFluor™ 520 can be excited by the blue (488nm) laser line and has a peak emission of 522nm that can be detected using a 530/30 band pass filter (equivalent to FITC)
  • For compensation, it is recommended to use a sample of the cells of interest stained with the Fixable Viability Dye
  • If the percentage of dead cells is expected to be less than 5%, then it is recommended to take a small aliquot of cells and heat them at 65°C for 1 minute then immediately place on ice for 1 minute. After this treatment, the heat-killed cells can be combined 1:1 with live cells and then stained with the Fixable Viability Dye.
  • Fixable Viability Dye eFluor™ 520 is supplied as a pre-diluted solution prepared in high-quality, anhydrous DMSO. It should be protected from light and moisture
  • Store at −70°C with dessicant
  • It may be freeze-thawed up to 20 times
  • Allow vial to equilibrate to room temperature before opening
  • Staining may be done before or after surface staining
  • Cells may be cryopreserved after staining with no adverse effect on staining intensity of dead cells after thawing
TRUSTED_SUSTAINABILITY

Specifications

Color Green
Detection Method Fluorescence
For Use With (Application) Viability Assay
For Use With (Equipment) Flow Cytometer
Product Type Dye
Emission 488/522
Product Line eBioscience
Quantity 500 tests

For Research Use Only. Not for use in diagnostic procedures.

Product Content Correction

Your input is important to us. Please complete this form to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Thank You! Your feedback has been submitted. Fisher Scientific is always working to improve our content for you. We appreciate your feedback.