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Description
RiboRuler Low Range RNA Ladder 100-1000 bp is a mixture of 7 chromatography purified single-stranded RNA transcripts (100-1000 bp long), produced from specific templates that contain a fragment of the pTZ19R polylinker and Lambda phage fragments.
Ladder is available in 1 mM EDTA, pH 6.0.
RiboRuler Low Range RNA Ladder is composed of chromatography-purified transcripts what makes them free from NTPs and RNA degradation products. The concentration of each RNA transcript is determined spectrophotometrically (see Photo for precise quantities). To minimize the possibility of contamination during the use the ladder is aliquotted in smaller volumes.
RiboRuler Low Range RNA Ladder is optimal for end-labeling using T4 Polynucleotide Kinase and is ideal for Northern blots.
Highlights
- Sharp bands of uniform intensity
- Easy-to-remember band sizes and quantities
- Approximate RNA quantification by predetermined concentration of ladder bands
- Supplied with 2X RNA Loading Dye
- Stable for 6 months at -20°C
Specifications
Specifications
| Content And Storage | • 5 x 20 μL RiboRuler Low Range RNA Ladder (in 1 mM EDTA, pH 6.0); sufficient for 50 uses • 1 mL RNA Loading Dye Store at -70°C or at -20°C (up to 6 months). |
| Concentration | 2X |
| Gel Type | Agarose |
| Label or Dye | Xylene Cyanol FF, Bromophenol Blue, Ethidium Bromide |
| Size Range | 100 to 1000 bp |
| Quantity | 5 x 20 μL |
| Product Line | RiboRuler |
| Product Type | DNA Ladder |
Safety and Handling
- RiboRuler Low Range RNA Ladder
- Danger
- Carcinogenicity Category 2
- Reproductive toxicity Category 1B
- Specific target organ toxicity after repeated exposure Category 2
- H351-Suspected of causing cancer.
- H360-May damage fertility or the unborn child.
- H373-May cause damage to organs.
- P201-Obtain special instructions before use.
- P202-Do not handle until all safety precautions have been read and understood.
- P260-Do not breathe dust/fume/gas/mist/vapours/spray.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P308+P313-IF exposed or concerned: Get medical advice/attention.
- P314-Get medical advice/attention if you feel unwell.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
- MIXTURE LIST-Contains : Formamide
For Research Use Only. Not for use in diagnostic procedures.
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