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Thermo Scientific™ Genomic DNA Purification Kit

Isolate genomic DNA from various sources, including whole blood, serum, cell lines, bacterial cells, plant, and mammalian tissues, with this DNA precipitation system.

Brand:  Thermo Scientific™ K0512

116.20 EUR valid until 2024-03-30
Use promo code "21648" to get your promotional price.



Additional Details : CAS : 9048-46-8 Weight : 2.66260kg


Product Code. 10571031

  • 166.00 € / Each
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Description

Description

Genomic DNA Purification Kit is a simple and rapid system for high quality genomic DNA purification from various sources including: whole blood, serum, cell lines, bacterial cells, plant, and mammalian tissues.

The kit is based on selective detergent-mediated DNA precipitation from crude lysate. The entire procedure is rapid only 20 to 25 minutes with a typical yield of 2 to 10 μg genomic DNA from 0.2 mL of blood.

High molecular weight genomic DNA purified with the kit is suitable for direct use in all common molecular biology applications: PCR, FastDigest and conventional restriction digestion, cloning, DNA sequencing, and Southern blot analysis.

Highlights

  • Efficient—2 to 10 μg of genomic DNA from 0.2 mL of blood
  • Fast—procedure takes approximately 20 minutes
  • Universal—purifies genomic DNA from various sources
  • Flexible—easy to scale up and down
  • Safe—does not involve phenol extraction
Specifications

Specifications

• 40 mL Lysis Solution; room temperature
• 8 mL Precipitation Solution (concentrated); room temperature
• 10 mL NaCl Solution; room temperature

Bacteria, Cells, Plant, Serum, Tissue, Whole Blood, Blood
gDNA
Not High-throughput Compatible (Manual)
Room Temperature
Bacteria: ≤20 mg
Cells: ≤106
Plant: ≤100 mg
Tissue: ≤30 mg
Serum: ≤200 μL
Whole Blood: ≤200 μL
Organic Extraction
20 to 100 μL
PCR, Southern blotting, sequencing, nucleic acid labeling, hybridization
100 Preps
25 min.
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Safety and Handling

Safety and Handling

Product Identifier
  • Genomic DNA Purification Kit

Signal Word
  • Danger

Hazard Category
  • LONG-TERM AQUATIC HAZARD Chronic 2
  • ACUTE AQUATIC Acute 1
  • Serious eye damage/eye irritation Category 1
  • Skin corrosion/irritation Category 2
  • Specific target organ toxicity after repeated exposure Category 2
  • Specific target organ toxicity Category 3

Hazard Statement
  • H315-Causes skin irritation.
  • H318-Causes serious eye damage.
  • H335-May cause respiratory irritation.
  • H373-May cause damage to organs.
  • H400-Very toxic to aquatic life.
  • H411-Toxic to aquatic life with long lasting effects.

Precautionary Statement
  • P260-Do not breathe dust/fume/gas/mist/vapours/spray.
  • P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
  • P264-Wash hands thoroughly after handling.
  • P271-Use only outdoors or in a well-ventilated area.
  • P273-Avoid release to the environment.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P302+P352-IF ON SKIN: Wash with plenty of water/soap.
  • P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
  • P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
  • P310-Immediately call a POISON CENTER/doctor/.
  • P312-Call a POISON CENTER/doctor/if you feel unwell.
  • P314-Get medical advice/attention if you feel unwell.
  • P332+P313-If skin irritation occurs: Get medical advice/attention.
  • P391-Collect spillage.
  • P403+P233-Store in a well-ventilated place. Keep container tightly closed.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information
  • MIXTURE LIST-Contains : N,N,N-trimethyl-1-tetradecanaminium bromide, Hexadecyltrimethylammonium bromide

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For Research Use Only. Not for use in diagnostic procedures.