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Description
GeneRuler Low Range DNA Ladder is recommended for sizing and approximate quantification of a wide range of double-stranded DNA fragments on agarose or polyacrylamide gels. It is ideal for denaturing polyacrylamide gel electrophoresis. The ladder is a mixture of chromatography-purified individual DNA fragments.
GeneRuler Low Range DNA Ladder can be labeled radioactively with T4 Polynucleotide Kinase.
- Ideal for both DNA sizing and approximate quantification
- Ideal for denaturing polyacrylamide gel electrophoresis
- Sharp bands
- Bright reference bands (given in red)
- Supplied with loading dye for sample DNA
Specifications
Specifications
| Concentration | 6X |
| Gel Type | Agarose |
| Label or Dye | Xylene Cyanol FF, Bromophenol Blue, Orange G |
| Ready to Load | No |
| Size Range | 25 to 700 bp |
| Gel Compatibility | Agarose Gels |
| Quantity | 50 μg |
| Product Line | GeneRuler |
| Product Type | DNA Ladder |
Frequently Asked Questions (FAQs)
The GelRed or GelGreen nucleic acid dyes can be used with our DNA Ladders. Nucleic acid binding dyes can affect DNA migration during electrophoresis, therefore we highly recommend post-staining of gels. Please follow the gel staining protocol provided by the manufacturer of nucleic acid gel dyes.
For fast sizing and quantification of double-stranded DNA, we recommend using FastRuler DNA Ladders. These ladders are easily resolved in a short separation distance after an 8-14 min run.
Each of Thermo Scientific DNA and RNA ladders has a recommended voltage given in V/cm. Please refer to the gel picture in the product information sheet. V/cm stands for the amount of voltage recommended per cm of the distance between the two electrodes of the gel box (not gel length).
Thermo Scientific DNA and RNA ladders have 5′-phosphate and 3′-hydroxyl groups.
For Research Use Only. Not for use in diagnostic procedures.
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