missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Enzymes and Inhibitors
  4. Restriction Enzymes
  5. 10399519

Thermo Scientific™ TAE Buffer (Tris-acetate-EDTA) (50X)

Product Code. 10399519 Shop All Thermo Scientific Products
Change view
Click to view available options
Quantity:
1 L
Unit Size:
1 L
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Product Code. Quantity unitSize
10399519 1 L 1 L
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 options
This item is not returnable. View return policy
Product Code. 10399519 Supplier Thermo Scientific™ Supplier No. B49

Please to purchase this item. Need a web account? Register with us today!

This item is not returnable. View return policy

Description

Optimize electrophoresis of both genomic and large supercoiled DNA in agarose and polyacrylamide gels with Thermo Scientific™ 50X TAE Buffer (Tris-acetate-EDTA).

Use the Thermo Scientific 50X TAE Buffer (Tris-acetate-EDTA) for electrophoresis of nucleic acids in agarose and polyacrylamide gels. You can use this buffer for both genomic and large supercoiled DNA, and you can also use this as both a running and a gel preparation buffer.

Usage Recommendations

  • Use fresh 1X TAE both for the gel and for the electrophoresis run
  • To prepare 1X TAE buffer add 20ml of 50X TAE buffer to 980ml of deionized water and mix well

Quality Control

  • The absence of endo-, exodeoxyribonucleases and ribonucleases confirmed by appropriate quality tests

1X Composition

  • 40mM Tris
  • 20mM acetic acid
  • 1mM EDTA
  • pHof 50X TAE: 8.4

Recommended for:

Electrophoresis of nucleic acids in agarose and polyacrylamide gels; Used both as a running buffer and as a gel preparation buffer; Filtered through a 0.22μm membrane; Recommended for resolution of RNA and DNA fragments l.

Note:

TAE buffer has a relatively low buffering capacity, therefore periodic replacement of the buffer during prolonged electrophoresis is recommended.

TRUSTED_SUSTAINABILITY

Specifications

Concentration 50X
Quantity 1 L
Product Type TAE Buffer

Safety and Handling

compliance-icons
Product Identifier
  • TAE Buffer (Tris-acetate-EDTA) (50X)
 Signal Word
  • Warning
 Hazard Category
  • Specific target organ toxicity after repeated exposure Category 2
 Hazard Statement
  • H373-May cause damage to organs.
 Precautionary Statement
  • P260-Do not breathe dust/fume/gas/mist/vapours/spray.
  • P314-Get medical advice/attention if you feel unwell.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
 Supplemental information
  • MIXTURE LIST-Contains : acetic acid
SDS

For Research Use Only. Not for use in diagnostic procedures.

Product Title
Select an issue

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.