Thermo Scientific™ FastAP Thermosensitive Alkaline Phosphatase (1 U/μL)

Highlights

• Recombinant enzyme
• Fast dephosphorylation – 10 minutes at 37°C
• Fast and complete inactivation – 5 minutes at 75°C
• Simultaneous digestion and dephosphorylation of vector DNA
• 100% active in restriction enzyme and PCR buffers
• One protocol for all types of DNA ends:
  • 5’-overhangs
  • 3’-overhangs
  • blunt-ends
  • single nucleotides
• PCR clean-up in conjunction with Exo I
• Protein dephosphorylation

Applications

• Dephosphorylation of cloning vector DNA to prevent recircularization during ligation
• Simultaneous digestion and dephosphorylation of vector DNA
• PCR product clean-up: nucleotide degradation prior to sequencing of PCR product
• Dephosphorylation of nucleic acid 5'-termini prior to labeling with T4 Polynucleotide Kinase
• Other applications where dephosphorylation of DNA and RNA substrates is necessary
• Protein dephosphorylation

Note

• Binding of FastAP Thermosensitive Alkaline Phosphatase to DNA may result in a band shift in agarose gels. To avoid this, incubate samples with 6X DNA Loading Dye & SDS Solution at 65°C for 10 minutes, and chill on ice prior to electrophoresis.
• FastAP Thermosensitive Alkaline Phosphatase is active in all restriction enzyme buffers and may be added directly to digested DNA. Heat inactivation of the restriction enzyme before dephosphorylation reaction is not necessary.