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  5. Merck Immobilon Western HRP Chemiluminescence Substrates

Merck Immobilon Western HRP Chemiluminescence Substrates

Product Code. 12634645 Shop All Merck Products
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Quantity:
500 mL
Product Type:
HRP Substrate
Sensitivity:
∼1 to 3 pg
∼400 fg
∼6 pg
Unit Size:
500mL
Each
This item is not returnable. View return policy

Product Code. 12634645

Brand: Merck WBLUF0500

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This item is not returnable. View return policy

Description

Premixed, ready-to-use chemiluminescent reagents for detection of HRP-based Westerns

Horseradish peroxidase (HRP) catalyzes the oxidation of luminol in the presence of peroxide, releasing photons (chemiluminescence) that are captured on x-ray films or by CCD cameras. Most HRP substrates are plagued by quickly decaying signal and short shelf life, but Luminata substrates are formulated differently to include stabilizing reagents.

  • Consistent results with less pipetting error

The stabilizers added to Luminata substrates:

  • Prevent spontaneous oxidation of luminol by peroxide in the absence of HRP
  • Enhance the stability of both the luminol and peroxide, ensuring that Luminata substrates are stable for one year in their premixed format
  • Stabilize, strengthen, and prolong the chemiluminescence signal once HRP starts to catalyze oxidation

Three different sensitivities for a range of detection needs:

  • Immobilon Classico premixed reagent for everyday detection needs
  • Immobilon Crescendo premixed reagent for detection in the picogram range
  • Immobilon Forte premixed reagent for detection in the femtogram range
TRUSTED_SUSTAINABILITY

Specifications

Product Type HRP Substrate
For Use With (Application) Dot/Slot Blot
Quantity 500 mL
Sensitivity ∼400 fg
Content And Storage 18°C to 24°C
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