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Invitrogen™ Total Antioxidant Capacity (T-AOC) Colorimetric Assay Kit (FRAP method)

Product Code. 17992031
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96 tests
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Pack of 96
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17992031 96 tests Pack of 96
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Product Code. 17992031 Supplier Invitrogen™ Supplier No. EEA024

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These complete, ready-to-use kits include clear 96-well plate(s), other assay specific reagents, buffers, and a lot-specific technical data sheet.

The Total Antioxidant Capacity (T-AOC) Colorimetric Assay Kit (FRAP method) is a highly reliable and sensitive assay designed for the quantitative measurement of T-AOC in serum, plasma, tissues, cells, cell culture supernatant, saliva, and urine sample types. T-AOC refers to the overall ability of a biological sample to counteract oxidative stress by neutralizing reactive oxygen species (ROS) and other harmful free radicals. It represents the cumulative effect of both enzymatic and non-enzymatic antioxidants present in the sample.

The assay quantifies the ability of the sample to reduce ferric ions (Fe3+) to ferrous ions (Fe2+) using the ferric reducing antioxidant power (FRAP) method under acidic conditions. T-AOC can be measured using the absorbance value of the resulting blue solution at 593 nm.

Key features
• Simple and fast protocol—user-friendly, requiring minimal hands-on time and providing rapid results within a short incubation period
• Broad range of kits—across key analytes and targets within the most important metabolic pathways
• Consistent—strict quality control criteria to ensure reproducible and reliable results

TRUSTED_SUSTAINABILITY

Specifications

Assay Range 0.23–2 mmol Trolox Equiv./L
Assay Sensitivity 0.049 mmol/L
Content And Storage Store at 2–8°C.

Detection Method Colorimetric
Sample Type Cell Culture Supernatant, Plasma, Saliva, Serum, Tissue Homogenates, Urine, Whole Cells
For Use With (Equipment) Microplate Reader
Incubation Time 3-5 min.
Interassay CV 8.1
Intraassay CV 3.9
Quantity 96 tests
Sample Volume 5 μL
Shipping Condition Dry Ice, 2–8°C
Target Kit Named Total Antioxidant Capacity (T-AOC)
Total Assay Time 20 min.
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