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STEMCELL Technologies EasySep™ Human CD4+CD127lowCD25+ Regulatory T Cell Isolation Kit

Product Code. 16244871
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Product Code. 16244871 Supplier STEMCELL Technologies Supplier No. 18063

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Description

Immunomagnetic negative selection of human CD4+CD127lowCD25+ regulatory T cells

  • This kit isolates human CD4+CD127lowCD25+ regulatory T cells (Tregs) from fresh peripheral blood mononuclear cells (PBMCs) or leukapheresis samples
  • First, CD25+ cells are isolated by column-free immunomagnetic positive selection using EasySep™ Releasable RapidSpheres™
  • Then, bound magnetic particles are removed from the EasySep™-isolated CD25+ cells, and unwanted non-Tregs are targeted for depletion
  • The final isolated fraction contains highly purified CD4+CD127lowCD25+ cells that express high levels of FOXP3 and are immediately ready for downstream applications
  • An optional protocol allows for the isolation of CD4+CD25- responder T cells in parallel for use in functional studies
  • Following cell isolation with this EasySep™ kit, antibody complexes remain bound to the cell surface and may interact with Brilliant Violet™ antibody conjugates, polyethylene glycol-modified proteins, or other chemically related ligands

Specifications

Cell Type T Cells, T Cells, CD4+, T Cells, Regulatory
For Use With (Application) Cell Isolation
Quantity 1 x 109 cells
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