Invitrogen™ E2F1 Polyclonal Antibody

Description

Recommended positive controls: 293T, A431, H1299, HeLaS3, HepG2, Molt-4, Raji, NIH-3T3, E2F1-transfected 293T. Predicted reactivity: Mouse (96%), Rat (97%), Chicken (86%), Bovine (96%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.