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Macherey-Nagel™ DNA Set for NucleoBond™ RNA Soil
Description
NucleoBond™ RNA Soil kit with the DNA Set enables efficient isolation of high quality RNA and DNA from any kind of soil or sediment sample. Most soils contain relatively low amounts of RNA, compared to DNA. Therefore, the kit is designed with proven NucleoBond™ technology to enable high sample input and and pure nucleic acids.
- Anion exchange technology to optimize RNA and DNA yield and purity—suitable for metagenomic studies
- Combination of mechanical homogenization and chemical lysis allows processing of large samples
- Parallel preparation of RNA and DNA in one hour*
- Sample disruption is achieved with NucleoSpin™ Bead Tubes Type A, optimized Lysis Buffer, and Phenol:Chloroform:Isoamylalcohol
- Optional Buffer OPT enables RNA and DNA purification from mineralic and organic soil samples
* DNA isolation requires DNA Set which is sold separately from the base NucleoBond™ RNA Soil kit (man. part no. 740140.20)
Specifications
Specifications
| Format | Gravity-flow Column |
| Isolation Technology | Anion Exchange Chromatography Technology |
| Sample Type | Sediment, Soil |
| Efficiency | Binding capacity: 600 μg |
| Elution Volume | Elution volume: 100 μL |
| For Use With (Equipment) | NucleoBond RNA Soil kit; Centrifuge for microcentrifuge tubes |
| For Use With (Application) | DNA isolation from soil for metagenomic study |
| Includes | NucleoBond Soil Columns, NucleoSpin Bead Tubes Type A, NucleoSpin Finisher, buffers |
| No. of Reactions | 20 |
| Purity | A260/A280: 1.7 - 2.1 |
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