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Thermo Scientific™ DH5α Competent Cells; High Efficiency

Product Code. 16512160 Shop All Thermo Scientific Products
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Quantity:
20 Reactions
Unit Size:
20 reactions
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For Research Use Only. All usage must comply with product instructions.

Product Code. 16512160

Brand: Thermo Scientific™ EC0112

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130.15 EUR valid until 2025-12-31
BEST PRICE on Promo! Use promo code "24346" to get your promotional price.
This item is not returnable. View return policy

For Research Use Only. All usage must comply with product instructions.

Description

High efficiency, chemically competent E. coli cells ideal for construction of gene banks or generation of cDNA libraries using plasmid-derived vectors

  • The φ80dlacZΔM15 marker provides α-complementation of the β-galactosidase gene from pUC or similar vectors to allow blue/white colony screening on bacterial agar plates containing Bluo-Gal or X-Gal
  • RecA1 and endA1 mutations in DH5α cells improve insert stability and quality of the extracted plasmid DNA
  • High transformation efficiency: >1x109 cfu/μg pUC19 DNA
  • Suitable for routine and high-throughput cloning applications
  • Genetic markers that allow for blue/white colony screening
  • Convenient two reactions per vial packaging
  • S.O.C. Outgrowth medium included
  • DH5α Competent Cells are suitable for variety of applications requiring high transformation efficiency:
    • Efficient DNA cloning derived from PCR, cDNA-generation reactions
    • recA1 marker facilitates working with difficult to transform DNA
    • Ideal for generation of cDNA libraries using plasmid-derived vectors
    • Site-directed mutagenesis
  • Genotype: F– φ80lacZΔ M15 Δ (lacZYA-argF) U169 recA1 endA1 hsdR17 (rK– mK+) phoA supE44 λ- thi–1 gyrA96 relA1
TRUSTED_SUSTAINABILITY

Specifications

Description Chemically Competent
Contains F' Episome Lacks F' Episome
Content And Storage Dry Ice (-80°C)
Bacterial or Yeast Strain DH5α
Species E. coli
Format Tube
For Use With (Application) Cloning
Quantity 20 Reactions
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