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Macherey-Nagel™ Detoxification Buffer ERB (1000 mL)
Innovative detoxification buffer removes endotoxins and proteins from plasmid DNA for use in transfection. Replacement buffer solution for use with NucleoSpin™ Plasmid/96 Plasmid Transfection-grade kits.
Brand: Macherey-Nagel™ 740495.1000
Additional Details : Weight : 1.00000kg ??product.attributeSet.Transport?? : UN number : 1993 Chem class : 3 Pack group : III
Description
Endotoxins are co-purified during typical plasmid preparations from bacterial lysates. Since they interfere with eukaryotic cell survival, endotoxin reduction is essential prior to cell transfection. NucleoSpin™ Plasmid Transfection-grade kits are designed to reduce endotoxins from bacterial lysates.
- Purifies high-copy plasmids for transfection
- Novel technology to diminish endotoxin content (< 50 EU/μg DNA) for successful transfection
- Rapid purification from E. coli via automatic, manual, vacuum or centrifugation processing
- Ultra-fast procedure simplifies the work-flow
- High-throughput — 96-well plates allow for easy automation and supports plasmid screening
- Downstream applications: transfection of common cell lines (lipofection, electroporation)
- Also suitable for standard molecular biology application, such as cloning and sequencing
Specifications
| Colorless | |
| Triethanolamine; 2-Propanol; Water | |
| 1000 mL | |
| Alcoholic | |
| Soluble in Water | |
| Detoxification buffer ERB |
| NucleoSpin Plasmid/96 Plasmid Transfection-grade full and core kit | |
| 5.5 to 6.5 | |
| 102-71-6 | |
| Room Temperature (18°C to 25°C) | |
| Liquid |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- Buffer ERB (1000 mL)
Signal Word
- Warning
Hazard Category
- Serious eye damage/eye irritation Category 2
- Flammable liquid Category 3
- Specific target organ toxicity Category 3
Hazard Statement
- H226-Flammable liquid and vapour.
- H319-Causes serious eye irritation.
- H336-May cause drowsiness or dizziness.
Precautionary Statement
- P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
- P260-Do not breathe dust/fume/gas/mist/vapours/spray.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
Supplemental information
- MIXTURE LIST-Contains : triethanolamine, 2-propanol