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Macherey-Nagel™ Detoxification Buffer ERB (1000 mL)

Innovative detoxification buffer removes endotoxins and proteins from plasmid DNA for use in transfection. Replacement buffer solution for use with NucleoSpin™ Plasmid/96 Plasmid Transfection-grade kits.

Brand:  Macherey-Nagel™ 740495.1000

Additional Details : Weight : 1.00000kg ??product.attributeSet.Transport?? : UN number : 1993 Chem class : 3 Pack group : III

Product Code. 15840918

  • 468.00 € / 1000mL
Estimated Shipment: 04-07-2024
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Endotoxins are co-purified during typical plasmid preparations from bacterial lysates. Since they interfere with eukaryotic cell survival, endotoxin reduction is essential prior to cell transfection. NucleoSpin™ Plasmid Transfection-grade kits are designed to reduce endotoxins from bacterial lysates.

  • Purifies high-copy plasmids for transfection
  • Novel technology to diminish endotoxin content (< 50 EU/μg DNA) for successful transfection
  • Rapid purification from E. coli via automatic, manual, vacuum or centrifugation processing
  • Ultra-fast procedure simplifies the work-flow
  • High-throughput — 96-well plates allow for easy automation and supports plasmid screening
  • Downstream applications: transfection of common cell lines (lipofection, electroporation)
  • Also suitable for standard molecular biology application, such as cloning and sequencing


Triethanolamine; 2-Propanol; Water
1000 mL
Soluble in Water
Detoxification buffer ERB
NucleoSpin Plasmid/96 Plasmid Transfection-grade full and core kit
5.5 to 6.5
Room Temperature (18°C to 25°C)
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Safety and Handling

Safety and Handling

Product Identifier
  • Buffer ERB (1000 mL)

Signal Word
  • Warning

Hazard Category
  • Serious eye damage/eye irritation Category 2
  • Flammable liquid Category 3
  • Specific target organ toxicity Category 3

Hazard Statement
  • H226-Flammable liquid and vapour.
  • H319-Causes serious eye irritation.
  • H336-May cause drowsiness or dizziness.

Precautionary Statement
  • P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
  • P260-Do not breathe dust/fume/gas/mist/vapours/spray.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.

Supplemental information
  • MIXTURE LIST-Contains : triethanolamine, 2-propanol