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Invitrogen™ Cyclic GMP Competitive ELISA Kit

Product Code. 10711195
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96 Tests
Unit Size:
1 set
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10711195 96 Tests 1 set
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Product Code. 10711195 Supplier Invitrogen™ Supplier No. EMSCGMPL

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Description

Competitive ELISA

The cyclic GMP Competitive ELISA quantitates cGMP in cell or tissue lysates. The assay will exclusively recognize both natural and recombinant cyclic GMP. Principle of the method The cyclic GMP solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is inversely proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Guanosine cyclic 3′,5′-(hydrogen phosphate) is a guanine nucleotide containing one phosphate group which is esterified to the sugar moiety in both the 3′- and 5′-positions. Cyclic gmp is a cellular regulatory agent and has been described as a second messenger. Its levels increase in response to a variety of hormones, including acetylcholine, insulin, and oxytocin and it has been found to activate specific protein kinases. [National Center for Biotechnology Information. PubChem Compound Database; CID=24316].
TRUSTED_SUSTAINABILITY

Specifications

Assay Range 0.8-5000 pmol/mL
Assay Sensitivity 0.059 pmol/mL
Description cGMP Competitive ELISA Kit
Conjugate AP
Immunoassay Kit Format Colorimetric ELISA (p-NPP substrate) with Plates
Product Type ELISA Kit
Sample Type Cell Lysates, Supernatant, Tissue Homogenates
Specificity No significant cross-reaction with GMP, GTP, cAMP, AMP, ATP, cUMP, or CTP
Sufficient For 96 microplate wells
Target Species Human
For Use With (Equipment) Colorimetric Microplate Reader
Interassay CV 7.5%
Intraassay CV 5.5%
Kit Contents Pre-coated 96-well plate, Detection Antibody, AP Conjugate, Neutralizing Reagent, Triethylamine, Acetic Anhydride, Standard, 20X Wash Buffer, pNpp Substrate Solution, Stop Solution, Plate Sealer, 0.1M HCl, 30mL
Label or Dye AP
Measurement Absorbance at 405nm minus absorbance at 570nm
Precision Dependent upon sample type, level and assay
Quantity 96 Tests
Regulatory Status RUO
Sample Volume Cell Lysate,100 μL; Supernatant, 100 μL; Tissue Homogenate,100 μL
Storage Requirements -20°C
Target Human
Test Time 1 hr. 20 min.
Total Assay Time 3 hr.
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