missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Cell Analysis Products
  4. Cell Based Assays
  5. General Cell Based Assays
  6. 10616645

Thermo Scientific™ CellSensor™ NFAT HEK 293T DA Assay Kit

Product Code. 10616645
Click to view available options
Quantity:
1 plate
Unit Size:
Each
This item is not returnable. View return policy

Product Code. 10616645

Brand: Thermo Scientific™ K1455

Please to purchase this item. Need a web account? Register with us today!

This item is currently unavailable or has been discontinued.
View the product page for possible alternatives.
 View alternative products

This item is not returnable. View return policy

Description

The CellSensor™ NFAT HEK 293T DA (Division Arrested) cells contain a beta-lactamase reporter gene under the control of the Nuclear Factor of Activated T Cells (NFAT) response element stably integrated into HEK 293T cells.

The CellSensor™ NFAT HEK 293T DA (Division Arrested) cells contain a beta-lactamase reporter gene under the control of the Nuclear Factor of Activated T Cells (NFAT) response element stably integrated into HEK 293T cells. Fluorescence activated cell sorting (FACS) was used to isolate clones responsive to stimulation of the NFAT pathway by PMA (Phorbol 12-Myristate 13-Acetate) and Thapsagargin (Thaps). The cell line was validated for DMSO tolerance, cell number, stimulation time, substrate loading conditions, and EC50 concentration of Thaps at a fixed concentration of PMA. DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin C, and have no apparent toxicity or change in cellular signal transduction.

This product can serve as a negative control in screening assays performed with specific G-protein coupled receptor (GPCR) DA cells that were made from this same background or these cells can be used in other assays where a sensitive readout to intracellular changes in calcium are needed.

TRUSTED_SUSTAINABILITY

Specifications

Cell State Division-Arrested Cells
Content And Storage Each kit contains sufficient quantities of division arrested (DA) cells and substrate to assay 1 x 384-well plate. (Other materials are required separately; please refer to the protocol).

Includes:
• CellSensor™ NFAT HEK293T DA cells
• LiveBLAzer™-FRET B/G Loading Kit, 70 μg
• Solution D
• CellSensor™ HEK293T CHO-K1 Assay Protocol
• LiveBLAzer™-FRET B/G Loading Protocol
• Certificate of Analysis

The CellSensor™ NFAT HEK 293T DA cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
For Use With (Application) GPCRs, Signaling Pathway
Includes CellSensor™ NFAT HEK293T DA cells, LiveBLAzer™-FRET B/G Loading Kit 70 μg, Solution D, CellSensor™ HEK293T CHO-K1 Assay Protocol, LiveBLAzer™-FRET B/G Loading Protocol, Certificate of Analysis
Product Line CellSensor™
Quantity 1 plate
Type Division-Arrested Cells Assay Kit
System Type CellSensor™
Druggable Target Signaling Pathway

For Research Use Only. Not for use in diagnostic procedures.

Product Content Correction

Your input is important to us. Please complete this form to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Thank You! Your feedback has been submitted. Fisher Scientific is always working to improve our content for you. We appreciate your feedback.