Invitrogen™ CD90 (Thy-1) Monoclonal Antibody (G7), Functional Grade, eBioscience™

Description

Description: The G7 monoclonal antibody reacts with mouse CD90 also known as Thy-1, a GPI-linked membrane molecule. CD90 is expressed by early hematopoietic cells in the bone marrow, thymocytes and mature T cells as well as neurons. CD90 is involved in regulation of adhesion and signal transduction by T cells. G7 is reported to induce TCR-mediated proliferation of T cell and apoptosis of thymocytes. Applications Reported: The G7 antibody has been reported for use in flow cytometric analysis. G7 has also been reported in functional studies. Applications Tested: The G7 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Storage and handling: Use in a sterile environment. Filtration: 0.2 μm post-manufacturing filtered. Purity: Greater than 90%, as determined by SDS-PAGE. Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay. Aggregation: Less than 10%, as determined by HPLC.

CD90 (Thy 1) antigen is a GPI linked glycoprotein member of the Immunoglobulin super family. CD90 is expressed in murine T cells, thymocytes, neural cells, Kupffer's cells and fibroblasts. CD90 may play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain. CD90 can be used as a marker for a variety of stem cells and for the axonal processes of mature neurons. Structural study of CD90 led to the foundation of the Immunoglobulin superfamily, of which it is the smallest member, and led to the first biochemical description and characterization of a vertebrate GPI anchor. Diseases associated with CD90 dysfunction include nasopharyngeal carcinoma and thymoma.