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  6. CD90.2 (Thy-1.2) Monoclonal Antibody (53-2.1), Super Bright™ 600, eBioscience™, Invitrogen™

CD90.2 (Thy-1.2) Monoclonal Antibody (53-2.1), Super Bright™ 600, eBioscience™, Invitrogen™

Product Code. p-7145126
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100 μg
25 μg
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100µg
25µg
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16371434 25 μg 25µg
16381434 100 μg 100µg
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Product Code. 16371434 Supplier eBioscience Supplier No. 63090280

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Description

Rat Monoclonal Antibody

Description: The 53-2.1 monoclonal antibody reacts with mouse CD90.2 also known as Thy-1.2, a GPI-linked membrane molecule. CD90.2 is expressed by mouse thymocytes and mature T cells as well as neurons in CD90.2-expressing mouse strains. These strains include BALB/c, CBA, C3H, C57BL/6, C58/, SJL and others. Cells from CD90.1-expressing strains including PL and AKR do not stain with 53-2.1. CD90 is involved in regulation of adhesion and signal transduction by T cells. Applications Reported: This 53-2.1 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 53-2.1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This may be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Super Bright 600 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 600 nm. We recommend using a 610/20 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome. When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Staining Buffer (cat.

SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorochrome performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 405 nm; Emission: 600 nm; Laser: Violet Laser Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company. CD90 (Thy 1) antigen is a GPI linked glycoprotein member of the Immunoglobulin super family. It is expressed in murine T cells, thymocytes, neural cells, Kupffer′s cells and fibroblasts. Thy 1 may play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.
TRUSTED_SUSTAINABILITY

Specifications

Antigen CD90.2 (Thy-1.2)
Applications Flow Cytometry
Classification Monoclonal
Clone 53-2.1
Concentration 0.2 mg/ml
Conjugate Super Bright 600
Formulation PBS with BSA and 0.09% sodium azide; pH 7.2
Gene Thy1
Gene Accession No. P01831
Gene Symbols Thy1
Host Species Rat
Purification Method Affinity chromatography
Quantity 25 μg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 21838
Target Species Mouse
Content And Storage 4° C, store in dark, DO NOT FREEZE!
Product Type Antibody
Form Liquid
Isotype IgG2a κ
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