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  6. Invitrogen™ CD321 (F11R) Monoclonal Antibody (CSTEM27), Alexa Fluor™ 488, eBioscience™, Invitrogen™

Invitrogen™ CD321 (F11R) Monoclonal Antibody (CSTEM27), Alexa Fluor™ 488, eBioscience™, Invitrogen™

Product Code. 15845463
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15845463 100 Tests Pack of 100
15835463 25 Tests Pack of 25
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Product Code. 15845463 Supplier Invitrogen™ Supplier No. 53321942

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Description

Mouse Monoclonal Antibody

This monoclonal antibody CSTEM27 reacts with human CD321 (F11R, JAM-1, JAM-A). Applications Reported: This CSTEM27 antibody has been reported for use in immunocytochemical staining and flow cytometric analysis. This CSTEM27 antibody has been tested by immunocytochemistry of adherent cells. This can be used at less than or equal to 5 μg/mL. This CSTEM27 antibody has also been tested by flow cytometric analysis of human iPSCs. This may be used at 0.25 μg/test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser. Filtration: 0.2 μm post-manufacturing filtered.

CD321 (F11R, JAM-1, JAM-A) is a cell-surface adhesion molecule belonging to the Ig superfamily of receptors. CD321 is a 32-41 kD glycoprotein, possessing two extracellular Ig-like domains, a transmembrane region, and a short cytosolic tail. The protein assembles at the membrane as a homodimer, and can associate laterally with other membrane proteins including integrins. CD321 is expressed on a variety of cell types, including leukocytes, platelets, erythrocytes, stem cells, epithelial and endothelial cells, and plays a role in mediating cell-cell contact. CD321 can be found in close proximity to several components of epithelial tight junctions, including ZO-1 and occludin. CD321 is involved in the maintenance of epithelial and endothelial barriers, hematopoiesis, angiogenesis, and tumor proliferation, invasion, and metastasis.
TRUSTED_SUSTAINABILITY

Specifications

Antigen CD321 (F11R)
Applications Flow Cytometry, Immunocytochemistry
Classification Monoclonal
Clone CSTEM27
Concentration 5 μL/Test
Conjugate Alexa Fluor 488
Formulation PBS with BSA and 0.09% sodium azide; pH 7.2
Gene F11r
Gene Accession No. Q9Y624
Gene Alias 9130004G24; AA638916; BV11 antigen; CD321; ESTM33; F11 R; F11 receptor; F11 receptor/F11R; F11R; JAM; JAM1; JAM-1; JAMA; JAM-A; Jcam; Jcam1; junction cell adhesion molecule A; junction cell adhesion molecule1; junctional adhesion molecule 1; Junctional adhesion molecule A; KAT; Ly106; PAM-1; Platelet adhesion molecule 1; platelet F11 receptor; UNQ264/PRO301
Gene Symbols F11r
Host Species Mouse
Purification Method Affinity chromatography
Quantity 100 Tests
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 50848
Target Species Human
Content And Storage 4°C, store in dark, DO NOT FREEZE!
Product Type Antibody
Form Liquid
Isotype IgG2a κ
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