Thermo Scientific™ CaptureSelect™ hCG Affinity Matrix

Some features of the CaptureSelect hCG Affinity Matrix include:

• Selective for hCG, with cross-binding to LH, FSH, and TSH
• Enables efficient purification of recombinant gonadotropins with high yields and purity
• Offers excellent scalability
• Non–animal-derived

Human gonadotropins (hCG, LH, FSH, TSH) are glycoprotein hormones secreted by gonadotrope cells of the pituitary gland that are often produced as recombinant proteins. They have an identical alpha chain subunit that is recognized by the ligand. Consequently, the CaptureSelect hCG Affinity Matrix shows binding to all human gonadotropins. The high selectivity and yields obtained using CaptureSelect hCG Affinity Matrix enable a robust and efficient purification process for hCG with excellent purity obtained in one step.

Free of animal components

CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).

Main characteristics

• Matrix: agarose-based, aldehyde-activated
• Average particle size: 65 ± 10 μm
• Ligand: CaptureSelect hCG affinity ligand
• Ligand coupling method: aldehyde coupling via NH₂ residues of the ligand
• Binding capacity: approx. 4 mg/mL resin (determined with HCG)
• Elution conditions: 20 mM citric acid, pH 3.0
• Flow characteristics: 50–200 cm/h (up to 2 bar)
• Formulation buffer: 20% (v/v) ethanol