Thermo Scientific™ CaptureSelect™ FcXP MiniChrom™ Pre-packed Column

These pre-packed columns are ready to connect and have the following features:

• Dimensions: 0.8 x 2 cm (1 mL), 0.8 x 10 cm (5 mL)
• Direct connection to standard chromatography systems
• Packed with CaptureSelect FcXP Affinity Matrix, offering high dynamic binding capacity

The 1-mL columns can be used for quick screenings of application feasibility and lab scale purification on a convenient and easy-to-use pre-packed column. The 10-cm bed height of the 5-mL columns allows initial process development on a bench scale.

CaptureSelect FcXP Affinity Matrix recognizes the CH3 domain of all human IgG subclasses (IgG1, 2, 3, and 4), enabling purification of all IgG subclasses and Fc fusion proteins directly from complex source materials in a robust and efficient purification process with excellent purity obtained in a single step.

Features of the FcXP affinity matrix include:

• Mild elution, making it suitable for Fc fusion proteins
• One-step purification from crude material with high purity and yield
• Human specific, no binding to bovine antibodies
• Excellent scalability
• Non–animal-derived

Free of animal components

CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).

Main characteristics

• Matrix: agarose-based, epoxide-activated
• Average particle size: 65 ± 10 μm
• Ligand: CaptureSelect FcXP affinity ligand
• Ligand coupling: Epoxide coupling
• Binding capacity: 40-50 g IgG/L resin depending on flow rate, column height, and contact time
• Elution conditions: 20 mM citric acid or acetic acid, pH 3–4
• Column dimensions: 0.8 x 2 cm (1 mL), 0.8 x 10 cm (5 mL)
• Flow characteristics: 75–150 cm/h (up to 2 bar)
• Formulation buffer: 20% (v/v) ethanol