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Invitrogen™ Bovine/Sheep Cortisol ELISA Kit

Product Code. 18057484
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Quantity:
96 Tests
Unit Size:
Pack of 96
This item is not returnable. View return policy

Product Code. 18057484

Brand: Invitrogen™ EEL015

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This item is not returnable. View return policy

Description

ELISA

Cortisol is the most potent glucocorticoid produced by the human adrenal. It is synthesized from cholesterol and its production is stimulated by pituitary adrenocorticotropic hormone (ACTH) which is regulated by corticotropin releasing factor (CRF). ACTH and CRF secretions are inhibited by high cortisol levels in a negative feedback loop. In plasma a majority of cortisol is bound with high affinity to corticosteroid binding globulin (CBG or transcotin). Cortisol acts through specific intracellular receptors and affects numerous physiologic systems including immune function, glucose counter regulation, vascular tone, and bone metabolism.
TRUSTED_SUSTAINABILITY

Specifications

Assay Range 1.56 to 100 ng/mL
Assay Sensitivity 0.96 ng/mL
Conjugate HRP
Product Type ELISA
Sample Type Plasma, Serum, Urine
For Use With (Equipment) Colorimetric Microplate Reader
Interassay CV <10%
Intraassay CV <10
Kit Contents Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
Label or Dye HRP
Quantity 96 Tests
Regulatory Status RUO
Sample Volume Plasma 50 μL, Serum 50 μL, Urine 100 μL
Storage Requirements Refer to product documentation for component specific storage temperature.
Target Cortisol
Test Time 1 hrs 20 mins
Total Assay Time 3 hrs 30 mins
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